Intracellular delivery of quantum dots for live cell labeling and organelle tracking

Austin M. Derfus; Warren C.W. Chan; Sangeeta N. Bhatia

doi:10.1002/adma.200306111

Intracellular delivery of quantum dots for live cell labeling and organelle tracking

Austin M. Derfus^*, Warren C.W. Chan, Sangeeta N. Bhatia

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

537 Citations (Scopus)

Abstract

The enhanced delivery of quantum dots (QD) to live cell labeling and organelle tracking was analyzed using epifluorescent microscopy and flow cytometry. It was observed that endocytosis of QDs results in sequestration of QDs in endolysomal compartment. The efficient schemes to deliver QDs to cytoplasm of large population of cells include delivery of QD/cationic liposome complexes and electroportation. The ability to target QDs to subcellar sites was also observed using known localization sequences.

Original language	English
Pages (from-to)	961-966
Number of pages	6
Journal	Advanced Materials
Volume	16
Issue number	12
DOIs	https://doi.org/10.1002/adma.200306111
Publication status	Published - Jun 17 2004
Externally published	Yes

ASJC Scopus Subject Areas

General Materials Science
Mechanics of Materials
Mechanical Engineering

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10.1002/adma.200306111

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abstract = "The enhanced delivery of quantum dots (QD) to live cell labeling and organelle tracking was analyzed using epifluorescent microscopy and flow cytometry. It was observed that endocytosis of QDs results in sequestration of QDs in endolysomal compartment. The efficient schemes to deliver QDs to cytoplasm of large population of cells include delivery of QD/cationic liposome complexes and electroportation. The ability to target QDs to subcellar sites was also observed using known localization sequences.",

author = "Derfus, {Austin M.} and Chan, {Warren C.W.} and Bhatia, {Sangeeta N.}",

year = "2004",

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AU - Derfus, Austin M.

AU - Chan, Warren C.W.

AU - Bhatia, Sangeeta N.

PY - 2004/6/17

Y1 - 2004/6/17

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AB - The enhanced delivery of quantum dots (QD) to live cell labeling and organelle tracking was analyzed using epifluorescent microscopy and flow cytometry. It was observed that endocytosis of QDs results in sequestration of QDs in endolysomal compartment. The efficient schemes to deliver QDs to cytoplasm of large population of cells include delivery of QD/cationic liposome complexes and electroportation. The ability to target QDs to subcellar sites was also observed using known localization sequences.

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DO - 10.1002/adma.200306111

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JO - Advanced Materials

JF - Advanced Materials

IS - 12

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Intracellular delivery of quantum dots for live cell labeling and organelle tracking

Abstract

ASJC Scopus Subject Areas

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