Abstract
In this study, the Saccharomyces cerevisiae wild type strain and engineered strain with an overexpressed heterologous ATP-citrate lyase (acl) were cultured in medium with different carbon and nitrogen concentrations, and their fatty acid production levels were investigated. The results showed that when the S. cerevisiae engineered strain was cultivated under nitrogen limited culture condition, the yield of mono-unsaturated fatty acids showed higher than that under non-nitrogen limited condition; with the carbon concentration increased, the accumulation become more apparent, whereas in the wild type strain, no such correlation was found. Besides, the citrate level in the S. cerevisiae under nitrogen limited condition was found to be much higher than that under non-nitrogen limited condition, which indicated a relationship between the diminution of nitrogen and accumulation of citrate in the S. cerevisiae. The accumulated citrate could be further cleaved by acl to provide substrate for fatty acid synthesis.
| Original language | English |
|---|---|
| Pages (from-to) | 200-206 |
| Number of pages | 7 |
| Journal | Bioresource Technology |
| Volume | 162 |
| DOIs | |
| Publication status | Published - Jun 2014 |
| Externally published | Yes |
ASJC Scopus Subject Areas
- Bioengineering
- Environmental Engineering
- Renewable Energy, Sustainability and the Environment
- Waste Management and Disposal
Keywords
- Engineered Saccharomyces cerevisiae
- Fatty acid accumulation
- Nitrogen limited culture condition
