Isolation of human peripheral blood T-lymphocytes

Atish Kizhakeyil; Seow Theng Ong; Mobashar Hussain Urf Turabe Fazil; Madhavi Latha Somaraju Chalasani; Praseetha Prasannan; Navin Kumar Verma

doi:10.1007/978-1-4939-9036-8_2

Isolation of human peripheral blood T-lymphocytes

Atish Kizhakeyil^*, Seow Theng Ong, Mobashar Hussain Urf Turabe Fazil, Madhavi Latha Somaraju Chalasani, Praseetha Prasannan, Navin Kumar Verma

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter

26 Citations (Scopus)

Abstract

Peripheral blood is the most common source of T-lymphocytes for in vitro culture. Here, we present a simple and standardized method for small- or large-scale isolation of viable T-lymphocytes and other mononuclear cells from fresh peripheral blood or buffy coat blood samples using the density gradient centrifugation. T-cells obtained using the protocol described here can be used for a variety of downstream analysis, including cellular, molecular, and functional assays.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	11-17
Number of pages	7
DOIs	https://doi.org/10.1007/978-1-4939-9036-8_2
Publication status	Published - 2019
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	1930
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© 2019, Springer Science+Business Media, LLC, part of Springer Nature.

ASJC Scopus Subject Areas

Molecular Biology
Genetics

Keywords

Density gradient
Ficoll
PBMC
T-Lymphocytes

Access to Document

10.1007/978-1-4939-9036-8_2

Cite this

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abstract = "Peripheral blood is the most common source of T-lymphocytes for in vitro culture. Here, we present a simple and standardized method for small- or large-scale isolation of viable T-lymphocytes and other mononuclear cells from fresh peripheral blood or buffy coat blood samples using the density gradient centrifugation. T-cells obtained using the protocol described here can be used for a variety of downstream analysis, including cellular, molecular, and functional assays.",

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AU - Kizhakeyil, Atish

AU - Ong, Seow Theng

AU - Fazil, Mobashar Hussain Urf Turabe

AU - Chalasani, Madhavi Latha Somaraju

AU - Prasannan, Praseetha

AU - Verma, Navin Kumar

PY - 2019

Y1 - 2019

N2 - Peripheral blood is the most common source of T-lymphocytes for in vitro culture. Here, we present a simple and standardized method for small- or large-scale isolation of viable T-lymphocytes and other mononuclear cells from fresh peripheral blood or buffy coat blood samples using the density gradient centrifugation. T-cells obtained using the protocol described here can be used for a variety of downstream analysis, including cellular, molecular, and functional assays.

AB - Peripheral blood is the most common source of T-lymphocytes for in vitro culture. Here, we present a simple and standardized method for small- or large-scale isolation of viable T-lymphocytes and other mononuclear cells from fresh peripheral blood or buffy coat blood samples using the density gradient centrifugation. T-cells obtained using the protocol described here can be used for a variety of downstream analysis, including cellular, molecular, and functional assays.

KW - Density gradient

KW - Ficoll

KW - PBMC

KW - T-Lymphocytes

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BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Isolation of human peripheral blood T-lymphocytes

Abstract

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Bibliographical note

ASJC Scopus Subject Areas

Keywords

Access to Document

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