Isolation of magnetically tagged cancer cells through an integrated magnetofluidic device

Qi Ying Wong; Ning Liu; Cheng Gee Koh; Hoi Yeung Li; Wen Siang Lew

doi:10.1007/s10404-016-1804-4

Isolation of magnetically tagged cancer cells through an integrated magnetofluidic device

Qi Ying Wong, Ning Liu, Cheng Gee Koh, Hoi Yeung Li, Wen Siang Lew^*

^*Corresponding author for this work

Nanyang Technological University

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Circulating tumour cells (CTC) in the bloodstream has been implicated in cancer metastasis. Efficient removal of CTC could potentially be an effective therapeutic measure against cancer metastasis. In this study, the hydrodynamic focusing flow in microfluidic channels (R_e ≪ 1) was considered together with the magnetophoretic force. The localised magnetic field was achieved through a passivated current-carrying multilayered microstripline, where the generated field gradient was used to attract the magnetic beads to the desired outlet. The experimental results show that the device is capable of isolating purely magnetic beads with an efficiency of 91 % while isolation efficiency of the magnetically tagged HeLa cervical cancer cells from cell suspension yielded an isolation efficiency of 79 %.

Original language	English
Article number	139
Journal	Microfluidics and Nanofluidics
Volume	20
Issue number	10
DOIs	https://doi.org/10.1007/s10404-016-1804-4
Publication status	Published - Oct 1 2016
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2016, Springer-Verlag Berlin Heidelberg.

ASJC Scopus Subject Areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Materials Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s10404-016-1804-4

Cite this

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abstract = "Circulating tumour cells (CTC) in the bloodstream has been implicated in cancer metastasis. Efficient removal of CTC could potentially be an effective therapeutic measure against cancer metastasis. In this study, the hydrodynamic focusing flow in microfluidic channels (Re ≪ 1) was considered together with the magnetophoretic force. The localised magnetic field was achieved through a passivated current-carrying multilayered microstripline, where the generated field gradient was used to attract the magnetic beads to the desired outlet. The experimental results show that the device is capable of isolating purely magnetic beads with an efficiency of 91 % while isolation efficiency of the magnetically tagged HeLa cervical cancer cells from cell suspension yielded an isolation efficiency of 79 %.",

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T1 - Isolation of magnetically tagged cancer cells through an integrated magnetofluidic device

AU - Wong, Qi Ying

AU - Liu, Ning

AU - Koh, Cheng Gee

AU - Li, Hoi Yeung

AU - Lew, Wen Siang

PY - 2016/10/1

Y1 - 2016/10/1

N2 - Circulating tumour cells (CTC) in the bloodstream has been implicated in cancer metastasis. Efficient removal of CTC could potentially be an effective therapeutic measure against cancer metastasis. In this study, the hydrodynamic focusing flow in microfluidic channels (Re ≪ 1) was considered together with the magnetophoretic force. The localised magnetic field was achieved through a passivated current-carrying multilayered microstripline, where the generated field gradient was used to attract the magnetic beads to the desired outlet. The experimental results show that the device is capable of isolating purely magnetic beads with an efficiency of 91 % while isolation efficiency of the magnetically tagged HeLa cervical cancer cells from cell suspension yielded an isolation efficiency of 79 %.

AB - Circulating tumour cells (CTC) in the bloodstream has been implicated in cancer metastasis. Efficient removal of CTC could potentially be an effective therapeutic measure against cancer metastasis. In this study, the hydrodynamic focusing flow in microfluidic channels (Re ≪ 1) was considered together with the magnetophoretic force. The localised magnetic field was achieved through a passivated current-carrying multilayered microstripline, where the generated field gradient was used to attract the magnetic beads to the desired outlet. The experimental results show that the device is capable of isolating purely magnetic beads with an efficiency of 91 % while isolation efficiency of the magnetically tagged HeLa cervical cancer cells from cell suspension yielded an isolation efficiency of 79 %.

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Isolation of magnetically tagged cancer cells through an integrated magnetofluidic device

Abstract

Bibliographical note

ASJC Scopus Subject Areas

UN SDGs

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