Label-free, electrochemical detection of methicillin-resistant staphylococcus aureus DNA with reduced graphene oxide-modified electrodes

Zhijuan Wang; Juan Zhang; Peng Chen; Xiaozhu Zhou; Yanli Yang; Shixin Wu; Li Niu; Yu Han; Lianhui Wang; Freddy Boey; Qichun Zhang; Bo Liedberg; Hua Zhang

doi:10.1016/j.bios.2011.03.002

Label-free, electrochemical detection of methicillin-resistant staphylococcus aureus DNA with reduced graphene oxide-modified electrodes

Zhijuan Wang, Juan Zhang, Peng Chen, Xiaozhu Zhou, Yanli Yang, Shixin Wu, Li Niu, Yu Han, Lianhui Wang, Freddy Boey, Qichun Zhang, Bo Liedberg, Hua Zhang^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

200 Citations (Scopus)

Abstract

Reduced graphene oxide (rGO)-modified glassy carbon electrode is used to detect the methicillin-resistant Staphylococcus aureus (MRSA) DNA by using electrochemical impedance spectroscopy. Our experiments confirm that ssDNA, before and after hybridization with target DNA, are successfully anchored on the rGO surface. After the probe DNA, pre-adsorbed on rGO electrode, hybridizes with target DNA, the measured impedance increases dramatically. It provides a new method to detect DNA with high sensitivity (10^-13M, i.e., 100 fM) and selectivity.

Original language	English
Pages (from-to)	3881-3886
Number of pages	6
Journal	Biosensors and Bioelectronics
Volume	26
Issue number	9
DOIs	https://doi.org/10.1016/j.bios.2011.03.002
Publication status	Published - May 15 2011
Externally published	Yes

ASJC Scopus Subject Areas

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

Keywords

DNA
Graphene
Impedance spectroscopy
Label-free detection
Methicillin-resistant Staphylococcus aureus
Reduced graphene oxide

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10.1016/j.bios.2011.03.002

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Wang, Z., Zhang, J., Chen, P., Zhou, X., Yang, Y., Wu, S., Niu, L., Han, Y., Wang, L., Boey, F., Zhang, Q., Liedberg, B., & Zhang, H. (2011). Label-free, electrochemical detection of methicillin-resistant staphylococcus aureus DNA with reduced graphene oxide-modified electrodes. Biosensors and Bioelectronics, 26(9), 3881-3886. https://doi.org/10.1016/j.bios.2011.03.002

@article{35f3e70337554104ae427b6cc3a9a9bc,

title = "Label-free, electrochemical detection of methicillin-resistant staphylococcus aureus DNA with reduced graphene oxide-modified electrodes",

abstract = "Reduced graphene oxide (rGO)-modified glassy carbon electrode is used to detect the methicillin-resistant Staphylococcus aureus (MRSA) DNA by using electrochemical impedance spectroscopy. Our experiments confirm that ssDNA, before and after hybridization with target DNA, are successfully anchored on the rGO surface. After the probe DNA, pre-adsorbed on rGO electrode, hybridizes with target DNA, the measured impedance increases dramatically. It provides a new method to detect DNA with high sensitivity (10-13M, i.e., 100 fM) and selectivity.",

keywords = "DNA, Graphene, Impedance spectroscopy, Label-free detection, Methicillin-resistant Staphylococcus aureus, Reduced graphene oxide",

author = "Zhijuan Wang and Juan Zhang and Peng Chen and Xiaozhu Zhou and Yanli Yang and Shixin Wu and Li Niu and Yu Han and Lianhui Wang and Freddy Boey and Qichun Zhang and Bo Liedberg and Hua Zhang",

year = "2011",

month = may,

day = "15",

doi = "10.1016/j.bios.2011.03.002",

language = "English",

volume = "26",

pages = "3881--3886",

journal = "Biosensors and Bioelectronics",

issn = "0956-5663",

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number = "9",

}

Wang, Z, Zhang, J, Chen, P, Zhou, X, Yang, Y, Wu, S, Niu, L, Han, Y, Wang, L, Boey, F, Zhang, Q, Liedberg, B & Zhang, H 2011, 'Label-free, electrochemical detection of methicillin-resistant staphylococcus aureus DNA with reduced graphene oxide-modified electrodes', Biosensors and Bioelectronics, vol. 26, no. 9, pp. 3881-3886. https://doi.org/10.1016/j.bios.2011.03.002

TY - JOUR

T1 - Label-free, electrochemical detection of methicillin-resistant staphylococcus aureus DNA with reduced graphene oxide-modified electrodes

AU - Wang, Zhijuan

AU - Zhang, Juan

AU - Chen, Peng

AU - Zhou, Xiaozhu

AU - Yang, Yanli

AU - Wu, Shixin

AU - Niu, Li

AU - Han, Yu

AU - Wang, Lianhui

AU - Boey, Freddy

AU - Zhang, Qichun

AU - Liedberg, Bo

AU - Zhang, Hua

PY - 2011/5/15

Y1 - 2011/5/15

N2 - Reduced graphene oxide (rGO)-modified glassy carbon electrode is used to detect the methicillin-resistant Staphylococcus aureus (MRSA) DNA by using electrochemical impedance spectroscopy. Our experiments confirm that ssDNA, before and after hybridization with target DNA, are successfully anchored on the rGO surface. After the probe DNA, pre-adsorbed on rGO electrode, hybridizes with target DNA, the measured impedance increases dramatically. It provides a new method to detect DNA with high sensitivity (10-13M, i.e., 100 fM) and selectivity.

AB - Reduced graphene oxide (rGO)-modified glassy carbon electrode is used to detect the methicillin-resistant Staphylococcus aureus (MRSA) DNA by using electrochemical impedance spectroscopy. Our experiments confirm that ssDNA, before and after hybridization with target DNA, are successfully anchored on the rGO surface. After the probe DNA, pre-adsorbed on rGO electrode, hybridizes with target DNA, the measured impedance increases dramatically. It provides a new method to detect DNA with high sensitivity (10-13M, i.e., 100 fM) and selectivity.

KW - DNA

KW - Graphene

KW - Impedance spectroscopy

KW - Label-free detection

KW - Methicillin-resistant Staphylococcus aureus

KW - Reduced graphene oxide

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U2 - 10.1016/j.bios.2011.03.002

DO - 10.1016/j.bios.2011.03.002

M3 - Article

C2 - 21458255

AN - SCOPUS:79955482784

SN - 0956-5663

VL - 26

SP - 3881

EP - 3886

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

IS - 9

ER -

Label-free, electrochemical detection of methicillin-resistant staphylococcus aureus DNA with reduced graphene oxide-modified electrodes

Abstract

ASJC Scopus Subject Areas

Keywords

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