Abstract
In vitro human hematopoietic stem cells (HSC) erythroid cultures produce immature red blood cells (RBC) called reticulocytes, which are important for RBC production. However, HSC cultures contain a complex mixture of erythroblasts, reticulocytes and expelled cell nuclei with overlapping cell sizes. Here, we use high-throughput deterministic lateral displacement microfluidic sorting method which utilizes unique inverse-L pillar design for the isolation of highly deformable reticulocytes (98% purity). This technique open new possibilities for investigating different DLD pillar effects on cell sorting based on label-free biophysical parameters such as rigidity, cytoplasm viscosity or nucleus size.
| Original language | English |
|---|---|
| Title of host publication | 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 256-258 |
| Number of pages | 3 |
| ISBN (Electronic) | 9781510897571 |
| Publication status | Published - 2018 |
| Externally published | Yes |
| Event | 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, Taiwan, Province of China Duration: Nov 11 2018 → Nov 15 2018 |
Publication series
| Name | 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 |
|---|---|
| Volume | 1 |
Conference
| Conference | 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 |
|---|---|
| Country/Territory | Taiwan, Province of China |
| City | Kaohsiung |
| Period | 11/11/18 → 11/15/18 |
Bibliographical note
Publisher Copyright:Copyright© (2018) by Chemical and Biological Microsystems Society.All rights reserved.
ASJC Scopus Subject Areas
- General Chemistry
- Bioengineering
- Chemical Engineering (miscellaneous)
- Control and Systems Engineering
Keywords
- Blood
- Cell Sorting
- Deterministic Lateral Displacement
- Stem Cell