Labeling and tracking P2 purinergic receptors in living cells using ATP-conjugated quantum dots

Shan Jiang; Aiping Liu; Hongwei Duan; Jianchow Soo; Peng Chen

doi:10.1002/adfm.201100472

Labeling and tracking P2 purinergic receptors in living cells using ATP-conjugated quantum dots

Shan Jiang, Aiping Liu, Hongwei Duan^*, Jianchow Soo, Peng Chen

^*Corresponding author for this work

Nanyang Technological University

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

It has been increasingly recognized that adenosine-5-triphosphate (ATP) signaling through P2 purinergic receptors has a diverse influence on cell functions and human health. In this contribution, a simple and non-invasive method to specifically label P2 receptors using ATP-conjugated quantum dots (QDs) is demonstrated. In addition, the endocytosis, vesicular trafficking, and recycling of QD-labeled P2 receptors are studied under different physiological conditions using confocal and total internal reflection fluorescence microscopy. Specifically and readily labeled by ATP-conjugated quantum dots (QDs), the endocytosis, vesicular trafficking and recycling of P2 purinergic receptors was studied in live cells, demonstrating their involvement in neuronal differentiation and apoptosis.

Original language	English
Pages (from-to)	2776-2780
Number of pages	5
Journal	Advanced Functional Materials
Volume	21
Issue number	14
DOIs	https://doi.org/10.1002/adfm.201100472
Publication status	Published - Jul 22 2011
Externally published	Yes

ASJC Scopus Subject Areas

Electronic, Optical and Magnetic Materials
General Chemistry
Biomaterials
General Materials Science
Condensed Matter Physics
Electrochemistry

Keywords

ATP
purinergic receptors
quantum dots
total internal reflection fluorescence microscopy
trafficking

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/adfm.201100472

Cite this

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title = "Labeling and tracking P2 purinergic receptors in living cells using ATP-conjugated quantum dots",

abstract = "It has been increasingly recognized that adenosine-5-triphosphate (ATP) signaling through P2 purinergic receptors has a diverse influence on cell functions and human health. In this contribution, a simple and non-invasive method to specifically label P2 receptors using ATP-conjugated quantum dots (QDs) is demonstrated. In addition, the endocytosis, vesicular trafficking, and recycling of QD-labeled P2 receptors are studied under different physiological conditions using confocal and total internal reflection fluorescence microscopy. Specifically and readily labeled by ATP-conjugated quantum dots (QDs), the endocytosis, vesicular trafficking and recycling of P2 purinergic receptors was studied in live cells, demonstrating their involvement in neuronal differentiation and apoptosis.",

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author = "Shan Jiang and Aiping Liu and Hongwei Duan and Jianchow Soo and Peng Chen",

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journal = "Advanced Functional Materials",

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TY - JOUR

T1 - Labeling and tracking P2 purinergic receptors in living cells using ATP-conjugated quantum dots

AU - Jiang, Shan

AU - Liu, Aiping

AU - Duan, Hongwei

AU - Soo, Jianchow

AU - Chen, Peng

PY - 2011/7/22

Y1 - 2011/7/22

N2 - It has been increasingly recognized that adenosine-5-triphosphate (ATP) signaling through P2 purinergic receptors has a diverse influence on cell functions and human health. In this contribution, a simple and non-invasive method to specifically label P2 receptors using ATP-conjugated quantum dots (QDs) is demonstrated. In addition, the endocytosis, vesicular trafficking, and recycling of QD-labeled P2 receptors are studied under different physiological conditions using confocal and total internal reflection fluorescence microscopy. Specifically and readily labeled by ATP-conjugated quantum dots (QDs), the endocytosis, vesicular trafficking and recycling of P2 purinergic receptors was studied in live cells, demonstrating their involvement in neuronal differentiation and apoptosis.

AB - It has been increasingly recognized that adenosine-5-triphosphate (ATP) signaling through P2 purinergic receptors has a diverse influence on cell functions and human health. In this contribution, a simple and non-invasive method to specifically label P2 receptors using ATP-conjugated quantum dots (QDs) is demonstrated. In addition, the endocytosis, vesicular trafficking, and recycling of QD-labeled P2 receptors are studied under different physiological conditions using confocal and total internal reflection fluorescence microscopy. Specifically and readily labeled by ATP-conjugated quantum dots (QDs), the endocytosis, vesicular trafficking and recycling of P2 purinergic receptors was studied in live cells, demonstrating their involvement in neuronal differentiation and apoptosis.

KW - ATP

KW - purinergic receptors

KW - quantum dots

KW - total internal reflection fluorescence microscopy

KW - trafficking

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DO - 10.1002/adfm.201100472

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ER -

Labeling and tracking P2 purinergic receptors in living cells using ATP-conjugated quantum dots

Abstract

ASJC Scopus Subject Areas

Keywords

UN SDGs

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