Layer-by-layer assembly of DNA-dye complex films

Xiaodong Chen; Jie Lang; Minghua Liu

doi:10.1016/S0040-6090(02)00045-7

Layer-by-layer assembly of DNA-dye complex films

Xiaodong Chen, Jie Lang, Minghua Liu^*

^*Corresponding author for this work

CAS - Institute of Chemistry

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

Complex films of 5,10,15,20-tetrakis(4-N-methylpyridyl)porphine tetra(p-toluenesulfonate) (TMPyP) and deoxyribonucleic acid (DNA) were formed by sequential deposition of TMPyP and DNA from aqueous solution onto quartz substrates. The deposition was monitored by absorption spectroscopy. It revealed that the DNA-TMPyP complex film is uniformly assembled layer-by-layer and the net charge of the dye is important in forming the DNA-dye film. The resulting DNA-dye complex multilayer films were characterized by UV-vis spectroscopy, fluorescence spectroscopy and fluorescence lifetime measurements. It has been found that TMPyP can either bind to the DNA electrostatically or intercalate into the base pairs. The relative ratio of the TMPyP intercalating into the DNA in the film is smaller than that in solution.

Original language	English
Pages (from-to)	227-232
Number of pages	6
Journal	Thin Solid Films
Volume	409
Issue number	2
DOIs	https://doi.org/10.1016/S0040-6090(02)00045-7
Publication status	Published - Apr 30 2002
Externally published	Yes

ASJC Scopus Subject Areas

Electronic, Optical and Magnetic Materials
Surfaces and Interfaces
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

Keywords

DNA
Dye
Layer-by-layer
Multilayers

Access to Document

10.1016/S0040-6090(02)00045-7

Cite this

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title = "Layer-by-layer assembly of DNA-dye complex films",

abstract = "Complex films of 5,10,15,20-tetrakis(4-N-methylpyridyl)porphine tetra(p-toluenesulfonate) (TMPyP) and deoxyribonucleic acid (DNA) were formed by sequential deposition of TMPyP and DNA from aqueous solution onto quartz substrates. The deposition was monitored by absorption spectroscopy. It revealed that the DNA-TMPyP complex film is uniformly assembled layer-by-layer and the net charge of the dye is important in forming the DNA-dye film. The resulting DNA-dye complex multilayer films were characterized by UV-vis spectroscopy, fluorescence spectroscopy and fluorescence lifetime measurements. It has been found that TMPyP can either bind to the DNA electrostatically or intercalate into the base pairs. The relative ratio of the TMPyP intercalating into the DNA in the film is smaller than that in solution.",

keywords = "DNA, Dye, Layer-by-layer, Multilayers",

author = "Xiaodong Chen and Jie Lang and Minghua Liu",

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doi = "10.1016/S0040-6090(02)00045-7",

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TY - JOUR

T1 - Layer-by-layer assembly of DNA-dye complex films

AU - Chen, Xiaodong

AU - Lang, Jie

AU - Liu, Minghua

PY - 2002/4/30

Y1 - 2002/4/30

N2 - Complex films of 5,10,15,20-tetrakis(4-N-methylpyridyl)porphine tetra(p-toluenesulfonate) (TMPyP) and deoxyribonucleic acid (DNA) were formed by sequential deposition of TMPyP and DNA from aqueous solution onto quartz substrates. The deposition was monitored by absorption spectroscopy. It revealed that the DNA-TMPyP complex film is uniformly assembled layer-by-layer and the net charge of the dye is important in forming the DNA-dye film. The resulting DNA-dye complex multilayer films were characterized by UV-vis spectroscopy, fluorescence spectroscopy and fluorescence lifetime measurements. It has been found that TMPyP can either bind to the DNA electrostatically or intercalate into the base pairs. The relative ratio of the TMPyP intercalating into the DNA in the film is smaller than that in solution.

AB - Complex films of 5,10,15,20-tetrakis(4-N-methylpyridyl)porphine tetra(p-toluenesulfonate) (TMPyP) and deoxyribonucleic acid (DNA) were formed by sequential deposition of TMPyP and DNA from aqueous solution onto quartz substrates. The deposition was monitored by absorption spectroscopy. It revealed that the DNA-TMPyP complex film is uniformly assembled layer-by-layer and the net charge of the dye is important in forming the DNA-dye film. The resulting DNA-dye complex multilayer films were characterized by UV-vis spectroscopy, fluorescence spectroscopy and fluorescence lifetime measurements. It has been found that TMPyP can either bind to the DNA electrostatically or intercalate into the base pairs. The relative ratio of the TMPyP intercalating into the DNA in the film is smaller than that in solution.

KW - DNA

KW - Dye

KW - Layer-by-layer

KW - Multilayers

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DO - 10.1016/S0040-6090(02)00045-7

M3 - Article

AN - SCOPUS:0037197355

SN - 0040-6090

VL - 409

SP - 227

EP - 232

JO - Thin Solid Films

JF - Thin Solid Films

IS - 2

ER -

Layer-by-layer assembly of DNA-dye complex films

Abstract

ASJC Scopus Subject Areas

Keywords

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