LC-MS based proteomic analysis for biofuel production

LC-MS (liquid chromatography-mass spectrometry) based proteomic analysis approach is an important research platform for biofuel production since the biofuel production process is playing a more and more important role in modern society and its pathways are complex which involves a large number of protein functions related to mysterious behaviors of cellular responses to the outside conditions. With its high resolution, sensitivity and separation efficiency of LC-MS analytical tool, a large number of proteins involved in different organisms and different cell lines can be identified. The protein functions explain the process of different biofuel production pathways. The different protein expression levels should describe better about the protein-protein relationships, the molecular level up-regulated or down-regulated mechanism and different impacts on the biofuel yields, such as nutrition stress, substrate specificity, etc. On the other hand, by qualifying and comparing to the different protein expression level during biofuel production, a better understanding of major enzymes' function taking part in the specific pathway can be achieved, which provides meaningful guidance for genetic modification of enzymes to either increase the biofuel production efficiency or improve the productive properties.