MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum

Aoli Xiong; Simran Kaushal; Ian Junjie Tay; Bevin P. Engelward; Jongyoon Han; Peter R. Preiser

doi:10.1016/j.xpro.2021.100797

MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum

Aoli Xiong, Simran Kaushal, Ian Junjie Tay, Bevin P. Engelward, Jongyoon Han, Peter R. Preiser^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Comet assay is a standard approach for studying DNA damage in malaria, but high-throughput options are not available. The CometChip was previously developed using mammalian cells as a high-throughput version of the comet assay. It is based on the same principle as the comet assay but provides greater efficacy, automated data processing, and improved consistency between experiments. In this protocol, we present MalariaCometChip to quantitatively assess drug-induced DNA damage in Plasmodium falciparum. For complete details on the use and execution of this protocol, please refer to Xiong et al. (2020).

Original language	English
Article number	100797
Journal	STAR Protocols
Volume	2
Issue number	3
DOIs	https://doi.org/10.1016/j.xpro.2021.100797
Publication status	Published - Sept 17 2021
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2021 The Authors

ASJC Scopus Subject Areas

General Neuroscience
General Biochemistry,Genetics and Molecular Biology
General Immunology and Microbiology

Keywords

Biotechnology and bioengineering
High-Throughput Screening
Microbiology
Microscopy
Molecular Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.xpro.2021.100797

Cite this

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title = "MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum",

abstract = "Comet assay is a standard approach for studying DNA damage in malaria, but high-throughput options are not available. The CometChip was previously developed using mammalian cells as a high-throughput version of the comet assay. It is based on the same principle as the comet assay but provides greater efficacy, automated data processing, and improved consistency between experiments. In this protocol, we present MalariaCometChip to quantitatively assess drug-induced DNA damage in Plasmodium falciparum. For complete details on the use and execution of this protocol, please refer to Xiong et al. (2020).",

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AU - Xiong, Aoli

AU - Kaushal, Simran

AU - Tay, Ian Junjie

AU - Engelward, Bevin P.

AU - Han, Jongyoon

AU - Preiser, Peter R.

PY - 2021/9/17

Y1 - 2021/9/17

N2 - Comet assay is a standard approach for studying DNA damage in malaria, but high-throughput options are not available. The CometChip was previously developed using mammalian cells as a high-throughput version of the comet assay. It is based on the same principle as the comet assay but provides greater efficacy, automated data processing, and improved consistency between experiments. In this protocol, we present MalariaCometChip to quantitatively assess drug-induced DNA damage in Plasmodium falciparum. For complete details on the use and execution of this protocol, please refer to Xiong et al. (2020).

AB - Comet assay is a standard approach for studying DNA damage in malaria, but high-throughput options are not available. The CometChip was previously developed using mammalian cells as a high-throughput version of the comet assay. It is based on the same principle as the comet assay but provides greater efficacy, automated data processing, and improved consistency between experiments. In this protocol, we present MalariaCometChip to quantitatively assess drug-induced DNA damage in Plasmodium falciparum. For complete details on the use and execution of this protocol, please refer to Xiong et al. (2020).

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KW - Microscopy

KW - Molecular Biology

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MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum

Abstract

Bibliographical note

ASJC Scopus Subject Areas

Keywords

UN SDGs

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