TY - JOUR
T1 - Metagenomics to paleogenomics
T2 - Large-scale sequencing of mammoth DNA
AU - Poinar, Hendrik N.
AU - Schwarz, Carsten
AU - Qi, Ji
AU - Shapiro, Beth
AU - MacPhee, Ross D.E.
AU - Buigues, Bernard
AU - Tikhonov, Alexei
AU - Huson, Daniel M.
AU - Tomsho, Lynn P.
AU - Auch, Alexander
AU - Rampp, Markus
AU - Miller, Webb
AU - Schuster, Stephan C.
PY - 2006/1/20
Y1 - 2006/1/20
N2 - We sequenced 28 million base pairs of DNA in a metagenomics approach, using a woolly mammoth (Mammuthus primigenius) sample from Siberia. As a result of exceptional sample preservation and the use of a recently developed emulsion polymerase chain reaction and pyrosequencing technique, 13 million base pairs (45.4%) of the sequencing reads were identified as mammoth DNA. Sequence identity between our data and African elephant (Loxodonta africana) was 98.55%, consistent with a paleontologically based divergence date of 5 to 6 million years. The sample includes a surprisingly small diversity of environmental DNAs. The high percentage of endogenous DNA recoverable from this single mammoth would allow for completion of its genome, unleashing the field of paleogenomics.
AB - We sequenced 28 million base pairs of DNA in a metagenomics approach, using a woolly mammoth (Mammuthus primigenius) sample from Siberia. As a result of exceptional sample preservation and the use of a recently developed emulsion polymerase chain reaction and pyrosequencing technique, 13 million base pairs (45.4%) of the sequencing reads were identified as mammoth DNA. Sequence identity between our data and African elephant (Loxodonta africana) was 98.55%, consistent with a paleontologically based divergence date of 5 to 6 million years. The sample includes a surprisingly small diversity of environmental DNAs. The high percentage of endogenous DNA recoverable from this single mammoth would allow for completion of its genome, unleashing the field of paleogenomics.
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U2 - 10.1126/science.1123360
DO - 10.1126/science.1123360
M3 - Article
C2 - 16368896
AN - SCOPUS:31144442151
SN - 0036-8075
VL - 311
SP - 392
EP - 394
JO - Science
JF - Science
IS - 5759
ER -