Microcapillary imaging of lamina cribrosa in porcine eyes using photoacoustic microscopy

Mohesh Moothanchery; Thanadet Chuangsuwanich; Alvan Tsz Chung Yan; Leopold Schmetterer; Michael J.A. Girard; Manojit Pramanik

doi:10.1117/12.2287825

Microcapillary imaging of lamina cribrosa in porcine eyes using photoacoustic microscopy

Mohesh Moothanchery, Thanadet Chuangsuwanich, Alvan Tsz Chung Yan, Leopold Schmetterer, Michael J.A. Girard, Manojit Pramanik

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

In order to understand the pathophysiology of glaucoma, Lamina cribrosa (LC) perfusion needs to be the subject of thorough investigation. It is currently difficult to obtain high resolution images of the embedded microcapillary network of the LC using conventional imaging techniques. In this study, an optical resolution photoacoustic microscopy (OR-PAM) system was used for imaging lamina cribrosa of an ex vivo porcine eye. Extrinsic contrast agent was used to perfuse the eye via its ciliary arteries. The OR-PAM system have a lateral resolution of 4 μm and an axial resolution of 30 μm. The high resolution system could able resolve a perfused LC microcapillary network to show vascular structure within the LC thickness. OR-PAM could be a promising imaging modality to study the LC perfusion and hence could be used to elucidate the hemodynamic aspect of glaucoma.

Original language	English
Title of host publication	Photons Plus Ultrasound
Subtitle of host publication	Imaging and Sensing 2018
Editors	Lihong V. Wang, Alexander A. Oraevsky
Publisher	SPIE
ISBN (Electronic)	9781510614734
DOIs	https://doi.org/10.1117/12.2287825
Publication status	Published - 2018
Externally published	Yes
Event	Photons Plus Ultrasound: Imaging and Sensing 2018 - San Francisco, United States Duration: Jan 28 2018 → Feb 1 2018

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	10494
ISSN (Print)	1605-7422

Conference

Conference	Photons Plus Ultrasound: Imaging and Sensing 2018
Country/Territory	United States
City	San Francisco
Period	1/28/18 → 2/1/18

Bibliographical note

Publisher Copyright:
© COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.

ASJC Scopus Subject Areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomaterials
Radiology Nuclear Medicine and imaging

Keywords

glaucoma
lamina cribrosa
microcapillary
optic nerve head
photoacoustic imaging

Access to Document

10.1117/12.2287825

Cite this

Moothanchery, M., Chuangsuwanich, T., Yan, A. T. C., Schmetterer, L., Girard, M. J. A., & Pramanik, M. (2018). Microcapillary imaging of lamina cribrosa in porcine eyes using photoacoustic microscopy. In L. V. Wang, & A. A. Oraevsky (Eds.), Photons Plus Ultrasound: Imaging and Sensing 2018 Article 104945Q (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10494). SPIE. https://doi.org/10.1117/12.2287825

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title = "Microcapillary imaging of lamina cribrosa in porcine eyes using photoacoustic microscopy",

abstract = "In order to understand the pathophysiology of glaucoma, Lamina cribrosa (LC) perfusion needs to be the subject of thorough investigation. It is currently difficult to obtain high resolution images of the embedded microcapillary network of the LC using conventional imaging techniques. In this study, an optical resolution photoacoustic microscopy (OR-PAM) system was used for imaging lamina cribrosa of an ex vivo porcine eye. Extrinsic contrast agent was used to perfuse the eye via its ciliary arteries. The OR-PAM system have a lateral resolution of 4 μm and an axial resolution of 30 μm. The high resolution system could able resolve a perfused LC microcapillary network to show vascular structure within the LC thickness. OR-PAM could be a promising imaging modality to study the LC perfusion and hence could be used to elucidate the hemodynamic aspect of glaucoma.",

keywords = "glaucoma, lamina cribrosa, microcapillary, optic nerve head, photoacoustic imaging",

author = "Mohesh Moothanchery and Thanadet Chuangsuwanich and Yan, \{Alvan Tsz Chung\} and Leopold Schmetterer and Girard, \{Michael J.A.\} and Manojit Pramanik",

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year = "2018",

doi = "10.1117/12.2287825",

language = "English",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

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Moothanchery, M, Chuangsuwanich, T, Yan, ATC, Schmetterer, L, Girard, MJA & Pramanik, M 2018, Microcapillary imaging of lamina cribrosa in porcine eyes using photoacoustic microscopy. in LV Wang & AA Oraevsky (eds), Photons Plus Ultrasound: Imaging and Sensing 2018., 104945Q, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 10494, SPIE, Photons Plus Ultrasound: Imaging and Sensing 2018, San Francisco, United States, 1/28/18. https://doi.org/10.1117/12.2287825

Microcapillary imaging of lamina cribrosa in porcine eyes using photoacoustic microscopy. / Moothanchery, Mohesh; Chuangsuwanich, Thanadet; Yan, Alvan Tsz Chung et al.
Photons Plus Ultrasound: Imaging and Sensing 2018. ed. / Lihong V. Wang; Alexander A. Oraevsky. SPIE, 2018. 104945Q (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10494).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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AU - Moothanchery, Mohesh

AU - Chuangsuwanich, Thanadet

AU - Yan, Alvan Tsz Chung

AU - Schmetterer, Leopold

AU - Girard, Michael J.A.

AU - Pramanik, Manojit

N1 - Publisher Copyright: © COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.

PY - 2018

Y1 - 2018

N2 - In order to understand the pathophysiology of glaucoma, Lamina cribrosa (LC) perfusion needs to be the subject of thorough investigation. It is currently difficult to obtain high resolution images of the embedded microcapillary network of the LC using conventional imaging techniques. In this study, an optical resolution photoacoustic microscopy (OR-PAM) system was used for imaging lamina cribrosa of an ex vivo porcine eye. Extrinsic contrast agent was used to perfuse the eye via its ciliary arteries. The OR-PAM system have a lateral resolution of 4 μm and an axial resolution of 30 μm. The high resolution system could able resolve a perfused LC microcapillary network to show vascular structure within the LC thickness. OR-PAM could be a promising imaging modality to study the LC perfusion and hence could be used to elucidate the hemodynamic aspect of glaucoma.

AB - In order to understand the pathophysiology of glaucoma, Lamina cribrosa (LC) perfusion needs to be the subject of thorough investigation. It is currently difficult to obtain high resolution images of the embedded microcapillary network of the LC using conventional imaging techniques. In this study, an optical resolution photoacoustic microscopy (OR-PAM) system was used for imaging lamina cribrosa of an ex vivo porcine eye. Extrinsic contrast agent was used to perfuse the eye via its ciliary arteries. The OR-PAM system have a lateral resolution of 4 μm and an axial resolution of 30 μm. The high resolution system could able resolve a perfused LC microcapillary network to show vascular structure within the LC thickness. OR-PAM could be a promising imaging modality to study the LC perfusion and hence could be used to elucidate the hemodynamic aspect of glaucoma.

KW - glaucoma

KW - lamina cribrosa

KW - microcapillary

KW - optic nerve head

KW - photoacoustic imaging

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BT - Photons Plus Ultrasound

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PB - SPIE

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Y2 - 28 January 2018 through 1 February 2018

ER -

Microcapillary imaging of lamina cribrosa in porcine eyes using photoacoustic microscopy

Abstract

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Bibliographical note

ASJC Scopus Subject Areas

Keywords

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