TY - JOUR
T1 - Molecular organization of the xcp gene cluster in Pseudomonas putida
T2 - Absence of an xcpX (gspK) homologue
AU - De Groot, Arjan
AU - Gerritse, Gijs
AU - Tommassen, Jan
AU - Lazdunski, Andrée
AU - Filloux, Alain
PY - 1999/1/8
Y1 - 1999/1/8
N2 - A DNA fragment containing xcp (gsp) gene homologues, required for extracellular protein secretion by the general secretory pathway (GSP) in various Gram-negative bacteria, was cloned from Pseudomonas putida (Pp) strain WCS358 and sequenced. The results presented here and those previously reported (de Groot, A., Krijger, J.-J., Filloux, A., Tommassen, J., 1996. Characterization of type II protein secretion (xcp) genes in the plant growth-stimulating Pseudomonas putida, strain WCS358 Mol. Gen. Genet. 250, 491-504) complete the sequence of the xcp gene cluster of Pp. Unlike that of Pseudomonas aeruginosa (Pa), the xcp gene cluster of Pp contains a gspN homologue. More surprisingly, in contrast to all known gsp gene clusters, the xcpX (gspK) homologue is not found. In addition, genes flanking the xcp cluster of Pp are not related to those flanking the xcp genes of Pa. Overall, the xcp gene products of Pp are as much related to those of Pa as to gsp gene products of enterobacterial species, suggesting that the xcp clusters of Pp and Pa have evolved separately.
AB - A DNA fragment containing xcp (gsp) gene homologues, required for extracellular protein secretion by the general secretory pathway (GSP) in various Gram-negative bacteria, was cloned from Pseudomonas putida (Pp) strain WCS358 and sequenced. The results presented here and those previously reported (de Groot, A., Krijger, J.-J., Filloux, A., Tommassen, J., 1996. Characterization of type II protein secretion (xcp) genes in the plant growth-stimulating Pseudomonas putida, strain WCS358 Mol. Gen. Genet. 250, 491-504) complete the sequence of the xcp gene cluster of Pp. Unlike that of Pseudomonas aeruginosa (Pa), the xcp gene cluster of Pp contains a gspN homologue. More surprisingly, in contrast to all known gsp gene clusters, the xcpX (gspK) homologue is not found. In addition, genes flanking the xcp cluster of Pp are not related to those flanking the xcp genes of Pa. Overall, the xcp gene products of Pp are as much related to those of Pa as to gsp gene products of enterobacterial species, suggesting that the xcp clusters of Pp and Pa have evolved separately.
KW - General secretory pathway
KW - Genomic organization
KW - GspN
KW - Protein secretion
KW - Pseudomonas aeruginosa
UR - http://www.scopus.com/inward/record.url?scp=0033534794&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033534794&partnerID=8YFLogxK
U2 - 10.1016/S0378-1119(98)00570-8
DO - 10.1016/S0378-1119(98)00570-8
M3 - Article
C2 - 9889311
AN - SCOPUS:0033534794
SN - 0378-1119
VL - 226
SP - 35
EP - 40
JO - Gene
JF - Gene
IS - 1
ER -