TY - JOUR
T1 - Multiple sensors control reciprocal expression of Pseudomonas aeruginosa regulatory RNA and virulence genes
AU - Ventre, Isabelle
AU - Goodman, Andrew L.
AU - Vallet-Gely, Isabelle
AU - Vasseur, Perrine
AU - Soscia, Chantal
AU - Molin, Søren
AU - Bleves, Sophie
AU - Lazdunski, Andrée
AU - Lory, Stephen
AU - Filloux, Alain
PY - 2006/1/3
Y1 - 2006/1/3
N2 - The opportunistic pathogen Pseudomonas aeruginosa is responsible for a wide range of acute and chronic infections. The transition to chronic infections is accompanied by physiological changes in the bacteria favoring formation of biofilm communities. Here we report the identification of LadS, a hybrid sensor kinase that controls the reciprocal expression of genes for type III secretion and biofilm-promoting polysaccharides. Domain organization of LadS and the range of LadS-controlled genes suggest that it counteracts the activities of another sensor kinase, RetS. These two pathways converge by controlling the transcription of a small regulatory RNA, RsmZ. This work identifies a previously undescribed signal transduction network in which the activities of signal-receiving sensor kinases LadS, RetS, and GacS regulate expression of virulence genes associated with acute or chronic infection by transcriptional and posttranscriptional mechanisms.
AB - The opportunistic pathogen Pseudomonas aeruginosa is responsible for a wide range of acute and chronic infections. The transition to chronic infections is accompanied by physiological changes in the bacteria favoring formation of biofilm communities. Here we report the identification of LadS, a hybrid sensor kinase that controls the reciprocal expression of genes for type III secretion and biofilm-promoting polysaccharides. Domain organization of LadS and the range of LadS-controlled genes suggest that it counteracts the activities of another sensor kinase, RetS. These two pathways converge by controlling the transcription of a small regulatory RNA, RsmZ. This work identifies a previously undescribed signal transduction network in which the activities of signal-receiving sensor kinases LadS, RetS, and GacS regulate expression of virulence genes associated with acute or chronic infection by transcriptional and posttranscriptional mechanisms.
KW - Biofilm
KW - pel genes
KW - Small RNA
KW - Two-component system
KW - Type III secretion
UR - http://www.scopus.com/inward/record.url?scp=30444453242&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=30444453242&partnerID=8YFLogxK
U2 - 10.1073/pnas.0507407103
DO - 10.1073/pnas.0507407103
M3 - Article
C2 - 16373506
AN - SCOPUS:30444453242
SN - 0027-8424
VL - 103
SP - 171
EP - 176
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 1
ER -