Mussel-Inspired Modification of Nanofibers for REST siRNA Delivery: Understanding the Effects of Gene-Silencing and Substrate Topography on Human Mesenchymal Stem Cell Neuronal Commitment

Wei Ching Low; Pim On Rujitanaroj; Dong Keun Lee; Jinghao Kuang; Phillip B. Messersmith; Jerry Kok Yen Chan; Sing Yian Chew

doi:10.1002/mabi.201500101

Mussel-Inspired Modification of Nanofibers for REST siRNA Delivery: Understanding the Effects of Gene-Silencing and Substrate Topography on Human Mesenchymal Stem Cell Neuronal Commitment

Wei Ching Low, Pim On Rujitanaroj, Dong Keun Lee, Jinghao Kuang, Phillip B. Messersmith, Jerry Kok Yen Chan, Sing Yian Chew^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

In this study, we promote neuronal differentiation of human mesenchymal stem cells (MSCs) through scaffold-mediated sustained release of siRNA targeting RE-1 silencing transcription factor (REST). Poly (ε-caprolactone) nanofibers were surface modified with mussel inspired DOPA-melanin (DM) coating for adsorption of REST siRNA. DM modification increased siRNA-loading efficiency and reduced the initial burst release. Fiber alignment and DM modification enhanced REST knockdown efficiencies. Under non-specific differentiation condition, REST silencing and fiber topography enhanced MSC neuronal markers expressions and reduced glial cell commitment. Such scaffolds may find useful applications in enhancing MSCs neuronal differentiation under non-specific conditions such as an in vivo environment.

Original language	English
Pages (from-to)	1457-1468
Number of pages	12
Journal	Macromolecular Bioscience
Volume	15
Issue number	10
DOIs	https://doi.org/10.1002/mabi.201500101
Publication status	Published - Oct 1 2015
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

ASJC Scopus Subject Areas

Biotechnology
Bioengineering
Biomaterials
Polymers and Plastics
Materials Chemistry

Keywords

electrospinning
neural differentiation
neuronal differentiation
RE-1 silencing transcription factor
sustained release

Access to Document

10.1002/mabi.201500101

Cite this

Low, W. C., Rujitanaroj, P. O., Lee, D. K., Kuang, J., Messersmith, P. B., Chan, J. K. Y., & Chew, S. Y. (2015). Mussel-Inspired Modification of Nanofibers for REST siRNA Delivery: Understanding the Effects of Gene-Silencing and Substrate Topography on Human Mesenchymal Stem Cell Neuronal Commitment. Macromolecular Bioscience, 15(10), 1457-1468. https://doi.org/10.1002/mabi.201500101

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abstract = "In this study, we promote neuronal differentiation of human mesenchymal stem cells (MSCs) through scaffold-mediated sustained release of siRNA targeting RE-1 silencing transcription factor (REST). Poly (ε-caprolactone) nanofibers were surface modified with mussel inspired DOPA-melanin (DM) coating for adsorption of REST siRNA. DM modification increased siRNA-loading efficiency and reduced the initial burst release. Fiber alignment and DM modification enhanced REST knockdown efficiencies. Under non-specific differentiation condition, REST silencing and fiber topography enhanced MSC neuronal markers expressions and reduced glial cell commitment. Such scaffolds may find useful applications in enhancing MSCs neuronal differentiation under non-specific conditions such as an in vivo environment.",

keywords = "electrospinning, neural differentiation, neuronal differentiation, RE-1 silencing transcription factor, sustained release",

author = "Low, {Wei Ching} and Rujitanaroj, {Pim On} and Lee, {Dong Keun} and Jinghao Kuang and Messersmith, {Phillip B.} and Chan, {Jerry Kok Yen} and Chew, {Sing Yian}",

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doi = "10.1002/mabi.201500101",

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Low, WC, Rujitanaroj, PO, Lee, DK, Kuang, J, Messersmith, PB, Chan, JKY & Chew, SY 2015, 'Mussel-Inspired Modification of Nanofibers for REST siRNA Delivery: Understanding the Effects of Gene-Silencing and Substrate Topography on Human Mesenchymal Stem Cell Neuronal Commitment', Macromolecular Bioscience, vol. 15, no. 10, pp. 1457-1468. https://doi.org/10.1002/mabi.201500101

Mussel-Inspired Modification of Nanofibers for REST siRNA Delivery: Understanding the Effects of Gene-Silencing and Substrate Topography on Human Mesenchymal Stem Cell Neuronal Commitment. / Low, Wei Ching; Rujitanaroj, Pim On; Lee, Dong Keun et al.
In: Macromolecular Bioscience, Vol. 15, No. 10, 01.10.2015, p. 1457-1468.

Research output: Contribution to journal › Article › peer-review

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Mussel-Inspired Modification of Nanofibers for REST siRNA Delivery: Understanding the Effects of Gene-Silencing and Substrate Topography on Human Mesenchymal Stem Cell Neuronal Commitment

Abstract

Bibliographical note

ASJC Scopus Subject Areas

Keywords

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