Nanocapsules Produced by Nanoprecipitation of Designed Suckerin-Silk Fusion Proteins

Ricardo Ramos; Kenrick Koh; Bartosz Gabryelczyk; Luxiao Chai; Deepankumar Kanagavel; Xibo Yan; François Ganachaud; Ali Miserez; Julien Bernard

doi:10.1021/acsmacrolett.1c00171

Nanocapsules Produced by Nanoprecipitation of Designed Suckerin-Silk Fusion Proteins

Ricardo Ramos, Kenrick Koh, Bartosz Gabryelczyk, Luxiao Chai, Deepankumar Kanagavel, Xibo Yan, François Ganachaud, Ali Miserez^*, Julien Bernard^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Herein, we report on the precise design of a modular fusion protein amenable to the construction of nanocapsules by nanoprecipitation. The central squid suckerin-derived peptide block provides structural stability, whereas both termini from spider silk fibroins make the protein highly soluble at physiological pH, a critical requirement for the nanoprecipitation process. With this design, nanocapsules consisting of fusion protein shells and oily cores with sizes in the range of 190-250 nm are built in a straightforward manner.

Original language	English
Pages (from-to)	628-634
Number of pages	7
Journal	ACS Macro Letters
Volume	10
Issue number	5
DOIs	https://doi.org/10.1021/acsmacrolett.1c00171
Publication status	Published - May 18 2021
Externally published	Yes

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© 2021 American Chemical Society. All rights reserved.

ASJC Scopus Subject Areas

Organic Chemistry
Polymers and Plastics
Inorganic Chemistry
Materials Chemistry

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10.1021/acsmacrolett.1c00171

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abstract = "Herein, we report on the precise design of a modular fusion protein amenable to the construction of nanocapsules by nanoprecipitation. The central squid suckerin-derived peptide block provides structural stability, whereas both termini from spider silk fibroins make the protein highly soluble at physiological pH, a critical requirement for the nanoprecipitation process. With this design, nanocapsules consisting of fusion protein shells and oily cores with sizes in the range of 190-250 nm are built in a straightforward manner.",

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AU - Koh, Kenrick

AU - Gabryelczyk, Bartosz

AU - Chai, Luxiao

AU - Kanagavel, Deepankumar

AU - Yan, Xibo

AU - Ganachaud, François

AU - Miserez, Ali

AU - Bernard, Julien

PY - 2021/5/18

Y1 - 2021/5/18

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AB - Herein, we report on the precise design of a modular fusion protein amenable to the construction of nanocapsules by nanoprecipitation. The central squid suckerin-derived peptide block provides structural stability, whereas both termini from spider silk fibroins make the protein highly soluble at physiological pH, a critical requirement for the nanoprecipitation process. With this design, nanocapsules consisting of fusion protein shells and oily cores with sizes in the range of 190-250 nm are built in a straightforward manner.

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Nanocapsules Produced by Nanoprecipitation of Designed Suckerin-Silk Fusion Proteins

Abstract

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ASJC Scopus Subject Areas

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