Nanoparticles Bind to Endothelial Cells in Injured Blood Vessels via a Transient Protein Corona

Zachary P. Lin; Wayne Ngo; Stefan M. Mladjenovic; Jamie L.Y. Wu; Warren C.W. Chan

doi:10.1021/acs.nanolett.2c04501

Nanoparticles Bind to Endothelial Cells in Injured Blood Vessels via a Transient Protein Corona

Zachary P. Lin, Wayne Ngo, Stefan M. Mladjenovic, Jamie L.Y. Wu, Warren C.W. Chan^*

^*Corresponding author for this work

University of Toronto

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

Nanoparticles travel through blood vessels to reach disease sites, but the local environment they encounter may affect their surface chemistry and cellular interactions. Here, we found that as nanoparticles transit through injured blood vessels they may interact with a highly localized concentration of platelet factor 4 proteins released from activated platelets. The platelet factor 4 binds to the nanoparticle surface and interacts with heparan sulfate proteoglycans on endothelial cells, and induces uptake. Understanding nanoparticle interactions with blood proteins and endothelial cells during circulation is critical to optimizing their design for diseased tissue targeting and delivery.

Original language	English
Pages (from-to)	1003-1009
Number of pages	7
Journal	Nano Letters
Volume	23
Issue number	3
DOIs	https://doi.org/10.1021/acs.nanolett.2c04501
Publication status	Published - Feb 8 2023
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2023 American Chemical Society.

ASJC Scopus Subject Areas

Bioengineering
General Chemistry
General Materials Science
Condensed Matter Physics
Mechanical Engineering

Keywords

Delivery
Endothelial cells
Nanoparticles
Platelet factor 4
Protein Corona
Vessels

Access to Document

10.1021/acs.nanolett.2c04501

Cite this

@article{2291c44245f84faf8c878299c6baf635,

title = "Nanoparticles Bind to Endothelial Cells in Injured Blood Vessels via a Transient Protein Corona",

abstract = "Nanoparticles travel through blood vessels to reach disease sites, but the local environment they encounter may affect their surface chemistry and cellular interactions. Here, we found that as nanoparticles transit through injured blood vessels they may interact with a highly localized concentration of platelet factor 4 proteins released from activated platelets. The platelet factor 4 binds to the nanoparticle surface and interacts with heparan sulfate proteoglycans on endothelial cells, and induces uptake. Understanding nanoparticle interactions with blood proteins and endothelial cells during circulation is critical to optimizing their design for diseased tissue targeting and delivery.",

keywords = "Delivery, Endothelial cells, Nanoparticles, Platelet factor 4, Protein Corona, Vessels",

author = "Lin, {Zachary P.} and Wayne Ngo and Mladjenovic, {Stefan M.} and Wu, {Jamie L.Y.} and Chan, {Warren C.W.}",

note = "Publisher Copyright: {\textcopyright} 2023 American Chemical Society.",

year = "2023",

month = feb,

day = "8",

doi = "10.1021/acs.nanolett.2c04501",

language = "English",

volume = "23",

pages = "1003--1009",

journal = "Nano Letters",

issn = "1530-6984",

publisher = "American Chemical Society",

number = "3",

}

TY - JOUR

T1 - Nanoparticles Bind to Endothelial Cells in Injured Blood Vessels via a Transient Protein Corona

AU - Lin, Zachary P.

AU - Ngo, Wayne

AU - Mladjenovic, Stefan M.

AU - Wu, Jamie L.Y.

AU - Chan, Warren C.W.

PY - 2023/2/8

Y1 - 2023/2/8

N2 - Nanoparticles travel through blood vessels to reach disease sites, but the local environment they encounter may affect their surface chemistry and cellular interactions. Here, we found that as nanoparticles transit through injured blood vessels they may interact with a highly localized concentration of platelet factor 4 proteins released from activated platelets. The platelet factor 4 binds to the nanoparticle surface and interacts with heparan sulfate proteoglycans on endothelial cells, and induces uptake. Understanding nanoparticle interactions with blood proteins and endothelial cells during circulation is critical to optimizing their design for diseased tissue targeting and delivery.

AB - Nanoparticles travel through blood vessels to reach disease sites, but the local environment they encounter may affect their surface chemistry and cellular interactions. Here, we found that as nanoparticles transit through injured blood vessels they may interact with a highly localized concentration of platelet factor 4 proteins released from activated platelets. The platelet factor 4 binds to the nanoparticle surface and interacts with heparan sulfate proteoglycans on endothelial cells, and induces uptake. Understanding nanoparticle interactions with blood proteins and endothelial cells during circulation is critical to optimizing their design for diseased tissue targeting and delivery.

KW - Delivery

KW - Endothelial cells

KW - Nanoparticles

KW - Platelet factor 4

KW - Protein Corona

KW - Vessels

UR - http://www.scopus.com/inward/record.url?scp=85146874940&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85146874940&partnerID=8YFLogxK

U2 - 10.1021/acs.nanolett.2c04501

DO - 10.1021/acs.nanolett.2c04501

M3 - Article

C2 - 36692977

AN - SCOPUS:85146874940

SN - 1530-6984

VL - 23

SP - 1003

EP - 1009

JO - Nano Letters

JF - Nano Letters

IS - 3

ER -

Nanoparticles Bind to Endothelial Cells in Injured Blood Vessels via a Transient Protein Corona

Abstract

Bibliographical note

ASJC Scopus Subject Areas

Keywords

Access to Document

Other files and links

Cite this