Nanoparticles strengthen intracellular tension and retard cellular migration

Chor Yong Tay; Pingqiang Cai; Magdiel I. Setyawati; Wanru Fang; Lay Poh Tan; Catherine H.L. Hong; Xiaodong Chen; David Tai Leong

doi:10.1021/nl4032549

Nanoparticles strengthen intracellular tension and retard cellular migration

Chor Yong Tay, Pingqiang Cai, Magdiel I. Setyawati, Wanru Fang, Lay Poh Tan, Catherine H.L. Hong, Xiaodong Chen^*, David Tai Leong

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

203 Citations (Scopus)

Abstract

Nanoparticles can have profound effects on cell biology. Here, we show that after TiO₂, SiO₂, and hydroxyapatite nanoparticles treatment, TR146 epithelial cell sheet displayed slower migration. Cells after exposure to the nanoparticles showed increased cell contractility with significantly impaired wound healing capability however without any apparent cytotoxicity. We showed the mechanism is through nanoparticle-mediated massive disruption of the intracellular microtubule assembly, thereby triggering a positive feedback that promoted stronger substrate adhesions thus leading to limited cell motility.

Original language	English
Pages (from-to)	83-88
Number of pages	6
Journal	Nano Letters
Volume	14
Issue number	1
DOIs	https://doi.org/10.1021/nl4032549
Publication status	Published - Jan 8 2014
Externally published	Yes

ASJC Scopus Subject Areas

Bioengineering
General Chemistry
General Materials Science
Condensed Matter Physics
Mechanical Engineering

Keywords

cell migration
cell traction force
microtubules
nanobio interactions
Nanoparticles

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10.1021/nl4032549

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title = "Nanoparticles strengthen intracellular tension and retard cellular migration",

abstract = "Nanoparticles can have profound effects on cell biology. Here, we show that after TiO2, SiO2, and hydroxyapatite nanoparticles treatment, TR146 epithelial cell sheet displayed slower migration. Cells after exposure to the nanoparticles showed increased cell contractility with significantly impaired wound healing capability however without any apparent cytotoxicity. We showed the mechanism is through nanoparticle-mediated massive disruption of the intracellular microtubule assembly, thereby triggering a positive feedback that promoted stronger substrate adhesions thus leading to limited cell motility.",

keywords = "cell migration, cell traction force, microtubules, nanobio interactions, Nanoparticles",

author = "Tay, {Chor Yong} and Pingqiang Cai and Setyawati, {Magdiel I.} and Wanru Fang and Tan, {Lay Poh} and Hong, {Catherine H.L.} and Xiaodong Chen and Leong, {David Tai}",

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doi = "10.1021/nl4032549",

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T1 - Nanoparticles strengthen intracellular tension and retard cellular migration

AU - Tay, Chor Yong

AU - Cai, Pingqiang

AU - Setyawati, Magdiel I.

AU - Fang, Wanru

AU - Tan, Lay Poh

AU - Hong, Catherine H.L.

AU - Chen, Xiaodong

AU - Leong, David Tai

PY - 2014/1/8

Y1 - 2014/1/8

N2 - Nanoparticles can have profound effects on cell biology. Here, we show that after TiO2, SiO2, and hydroxyapatite nanoparticles treatment, TR146 epithelial cell sheet displayed slower migration. Cells after exposure to the nanoparticles showed increased cell contractility with significantly impaired wound healing capability however without any apparent cytotoxicity. We showed the mechanism is through nanoparticle-mediated massive disruption of the intracellular microtubule assembly, thereby triggering a positive feedback that promoted stronger substrate adhesions thus leading to limited cell motility.

AB - Nanoparticles can have profound effects on cell biology. Here, we show that after TiO2, SiO2, and hydroxyapatite nanoparticles treatment, TR146 epithelial cell sheet displayed slower migration. Cells after exposure to the nanoparticles showed increased cell contractility with significantly impaired wound healing capability however without any apparent cytotoxicity. We showed the mechanism is through nanoparticle-mediated massive disruption of the intracellular microtubule assembly, thereby triggering a positive feedback that promoted stronger substrate adhesions thus leading to limited cell motility.

KW - cell migration

KW - cell traction force

KW - microtubules

KW - nanobio interactions

KW - Nanoparticles

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Nanoparticles strengthen intracellular tension and retard cellular migration

Abstract

ASJC Scopus Subject Areas

Keywords

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