Osmium carbonyl clusters containing labile ligands hyperstabilize microtubules

Voon Kong Kien; Kee Leong Weng; Lina H.K. Lim

doi:10.1021/tx900056a

Osmium carbonyl clusters containing labile ligands hyperstabilize microtubules

Voon Kong Kien, Kee Leong Weng, Lina H.K. Lim

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

A study into the possible molecular targets of the osmium carbonyl cluster Os3(CO) ₁₀(NCCH ₃) ₂ (2) in the ER- breast carcinoma (MDA-MB-231) cell line was carried out. Infrared and ¹H NMR analyses of cells treated with 2 showed the formation of carboxylato- and thiolato-bridged clusters from the interaction with intracellular carboxylic acid and sulfhydryl residues. The cytotoxicity of 2 was reduced in the presence of fetal bovine serum, and measurement with Ellman's reagent as well as fluorescence confocal microscopy with tetramethylrhodamine-5-maleimide staining all demonstrated binding to intracellular sulfhydryl groups leading up to cell disruption. Tubulin-FITC antibody staining of treated cells showed disruption of the microtubules, and a tubulin polmerization assay showed that 2 induced hyperstabilization of the microtubules.

Original language	English
Pages (from-to)	1116-1122
Number of pages	7
Journal	Chemical Research in Toxicology
Volume	22
Issue number	6
DOIs	https://doi.org/10.1021/tx900056a
Publication status	Published - Jun 15 2009
Externally published	Yes

ASJC Scopus Subject Areas

Toxicology

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title = "Osmium carbonyl clusters containing labile ligands hyperstabilize microtubules",

abstract = "A study into the possible molecular targets of the osmium carbonyl cluster Os3(CO) 10(NCCH 3) 2 (2) in the ER- breast carcinoma (MDA-MB-231) cell line was carried out. Infrared and 1H NMR analyses of cells treated with 2 showed the formation of carboxylato- and thiolato-bridged clusters from the interaction with intracellular carboxylic acid and sulfhydryl residues. The cytotoxicity of 2 was reduced in the presence of fetal bovine serum, and measurement with Ellman's reagent as well as fluorescence confocal microscopy with tetramethylrhodamine-5-maleimide staining all demonstrated binding to intracellular sulfhydryl groups leading up to cell disruption. Tubulin-FITC antibody staining of treated cells showed disruption of the microtubules, and a tubulin polmerization assay showed that 2 induced hyperstabilization of the microtubules.",

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AU - Kien, Voon Kong

AU - Weng, Kee Leong

AU - Lim, Lina H.K.

PY - 2009/6/15

Y1 - 2009/6/15

N2 - A study into the possible molecular targets of the osmium carbonyl cluster Os3(CO) 10(NCCH 3) 2 (2) in the ER- breast carcinoma (MDA-MB-231) cell line was carried out. Infrared and 1H NMR analyses of cells treated with 2 showed the formation of carboxylato- and thiolato-bridged clusters from the interaction with intracellular carboxylic acid and sulfhydryl residues. The cytotoxicity of 2 was reduced in the presence of fetal bovine serum, and measurement with Ellman's reagent as well as fluorescence confocal microscopy with tetramethylrhodamine-5-maleimide staining all demonstrated binding to intracellular sulfhydryl groups leading up to cell disruption. Tubulin-FITC antibody staining of treated cells showed disruption of the microtubules, and a tubulin polmerization assay showed that 2 induced hyperstabilization of the microtubules.

AB - A study into the possible molecular targets of the osmium carbonyl cluster Os3(CO) 10(NCCH 3) 2 (2) in the ER- breast carcinoma (MDA-MB-231) cell line was carried out. Infrared and 1H NMR analyses of cells treated with 2 showed the formation of carboxylato- and thiolato-bridged clusters from the interaction with intracellular carboxylic acid and sulfhydryl residues. The cytotoxicity of 2 was reduced in the presence of fetal bovine serum, and measurement with Ellman's reagent as well as fluorescence confocal microscopy with tetramethylrhodamine-5-maleimide staining all demonstrated binding to intracellular sulfhydryl groups leading up to cell disruption. Tubulin-FITC antibody staining of treated cells showed disruption of the microtubules, and a tubulin polmerization assay showed that 2 induced hyperstabilization of the microtubules.

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Osmium carbonyl clusters containing labile ligands hyperstabilize microtubules

Abstract

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