Peptide functionalized gold nanoparticles for colorimetric detection of matrilysin (MMP-7) activity

Peng Chen; Robert Selegård; Daniel Aili; Bo Liedberg

doi:10.1039/c3nr03006g

Peptide functionalized gold nanoparticles for colorimetric detection of matrilysin (MMP-7) activity

Peng Chen, Robert Selegård, Daniel Aili, Bo Liedberg^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

71 Citations (Scopus)

Abstract

A peptide with two cleavage sites for MMP-7 has been synthesized and immobilized on gold nanoparticles (AuNPs) through a cysteine residue. Digestion of the peptide by MMP-7 decreases its size and net charge, which leads to the aggregation of the AuNPs. The color shift caused by aggregation enables a direct and quantitative measurement of the concentration and activity of MMP-7 with an estimated limit of detection of ∼5 nM (0.1 μg mL^-1).

Original language	English
Pages (from-to)	8973-8976
Number of pages	4
Journal	Nanoscale
Volume	5
Issue number	19
DOIs	https://doi.org/10.1039/c3nr03006g
Publication status	Published - Oct 7 2013
Externally published	Yes

ASJC Scopus Subject Areas

General Materials Science

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abstract = "A peptide with two cleavage sites for MMP-7 has been synthesized and immobilized on gold nanoparticles (AuNPs) through a cysteine residue. Digestion of the peptide by MMP-7 decreases its size and net charge, which leads to the aggregation of the AuNPs. The color shift caused by aggregation enables a direct and quantitative measurement of the concentration and activity of MMP-7 with an estimated limit of detection of ∼5 nM (0.1 μg mL-1).",

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AU - Chen, Peng

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AU - Aili, Daniel

AU - Liedberg, Bo

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Y1 - 2013/10/7

N2 - A peptide with two cleavage sites for MMP-7 has been synthesized and immobilized on gold nanoparticles (AuNPs) through a cysteine residue. Digestion of the peptide by MMP-7 decreases its size and net charge, which leads to the aggregation of the AuNPs. The color shift caused by aggregation enables a direct and quantitative measurement of the concentration and activity of MMP-7 with an estimated limit of detection of ∼5 nM (0.1 μg mL-1).

AB - A peptide with two cleavage sites for MMP-7 has been synthesized and immobilized on gold nanoparticles (AuNPs) through a cysteine residue. Digestion of the peptide by MMP-7 decreases its size and net charge, which leads to the aggregation of the AuNPs. The color shift caused by aggregation enables a direct and quantitative measurement of the concentration and activity of MMP-7 with an estimated limit of detection of ∼5 nM (0.1 μg mL-1).

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Peptide functionalized gold nanoparticles for colorimetric detection of matrilysin (MMP-7) activity

Abstract

ASJC Scopus Subject Areas

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