Peptide Functionalized Gold Nanoparticles with Optimized Particle Size and Concentration for Colorimetric Assay Development: Detection of Cardiac Troponin i

Xiaohu Liu; Yi Wang; Peng Chen; Austin McCadden; Alagappan Palaniappan; Jinling Zhang; Bo Liedberg

doi:10.1021/acssensors.6b00493

Peptide Functionalized Gold Nanoparticles with Optimized Particle Size and Concentration for Colorimetric Assay Development: Detection of Cardiac Troponin i

Xiaohu Liu, Yi Wang, Peng Chen, Austin McCadden, Alagappan Palaniappan, Jinling Zhang, Bo Liedberg^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

85 Citations (Scopus)

Abstract

Peptide-functionalized gold nanoparticles (AuNPs) are extensively utilized in colorimetric assays for rapid and sensitive detection of various biomedical and environmental targets. Although extensively used as colorimetric reporting systems, the role of the size and concentration of the AuNPs has not been thoroughly investigated. In this study, a 12-mer cardiac troponin I (cTnI)-specific peptide CALNN-Peg₄-FYSHSFHENWPS was immobilized on AuNPs of different size and concentration via the CALNN anchoring sequence. A relationship was established between the total surface area of the AuNPs (binding availability) and response (centroid shift). Moreover, a colorimetric assay for cTnI operating under optimized conditions (36 nm AuNPs) yielded a limit of detection of 0.2 ng/mL (8.4 pM) when tested in diluted serum samples with an assay time of 10 min. This encouraging result opens up for further development of AuNP assays in early diagnosis of cardiac injury.

Original language	English
Pages (from-to)	1416-1422
Number of pages	7
Journal	ACS Sensors
Volume	1
Issue number	12
DOIs	https://doi.org/10.1021/acssensors.6b00493
Publication status	Published - Dec 23 2016
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2016 American Chemical Society.

ASJC Scopus Subject Areas

Bioengineering
Instrumentation
Process Chemistry and Technology
Fluid Flow and Transfer Processes

Keywords

cardiac troponin I
colorimetric sensing
concentration of AuNPs
peptide-functionalized gold nanoparticles
size of AuNPs

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title = "Peptide Functionalized Gold Nanoparticles with Optimized Particle Size and Concentration for Colorimetric Assay Development: Detection of Cardiac Troponin i",

abstract = "Peptide-functionalized gold nanoparticles (AuNPs) are extensively utilized in colorimetric assays for rapid and sensitive detection of various biomedical and environmental targets. Although extensively used as colorimetric reporting systems, the role of the size and concentration of the AuNPs has not been thoroughly investigated. In this study, a 12-mer cardiac troponin I (cTnI)-specific peptide CALNN-Peg4-FYSHSFHENWPS was immobilized on AuNPs of different size and concentration via the CALNN anchoring sequence. A relationship was established between the total surface area of the AuNPs (binding availability) and response (centroid shift). Moreover, a colorimetric assay for cTnI operating under optimized conditions (36 nm AuNPs) yielded a limit of detection of 0.2 ng/mL (8.4 pM) when tested in diluted serum samples with an assay time of 10 min. This encouraging result opens up for further development of AuNP assays in early diagnosis of cardiac injury.",

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author = "Xiaohu Liu and Yi Wang and Peng Chen and Austin McCadden and Alagappan Palaniappan and Jinling Zhang and Bo Liedberg",

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T2 - Detection of Cardiac Troponin i

AU - Liu, Xiaohu

AU - Wang, Yi

AU - Chen, Peng

AU - McCadden, Austin

AU - Palaniappan, Alagappan

AU - Zhang, Jinling

AU - Liedberg, Bo

PY - 2016/12/23

Y1 - 2016/12/23

N2 - Peptide-functionalized gold nanoparticles (AuNPs) are extensively utilized in colorimetric assays for rapid and sensitive detection of various biomedical and environmental targets. Although extensively used as colorimetric reporting systems, the role of the size and concentration of the AuNPs has not been thoroughly investigated. In this study, a 12-mer cardiac troponin I (cTnI)-specific peptide CALNN-Peg4-FYSHSFHENWPS was immobilized on AuNPs of different size and concentration via the CALNN anchoring sequence. A relationship was established between the total surface area of the AuNPs (binding availability) and response (centroid shift). Moreover, a colorimetric assay for cTnI operating under optimized conditions (36 nm AuNPs) yielded a limit of detection of 0.2 ng/mL (8.4 pM) when tested in diluted serum samples with an assay time of 10 min. This encouraging result opens up for further development of AuNP assays in early diagnosis of cardiac injury.

AB - Peptide-functionalized gold nanoparticles (AuNPs) are extensively utilized in colorimetric assays for rapid and sensitive detection of various biomedical and environmental targets. Although extensively used as colorimetric reporting systems, the role of the size and concentration of the AuNPs has not been thoroughly investigated. In this study, a 12-mer cardiac troponin I (cTnI)-specific peptide CALNN-Peg4-FYSHSFHENWPS was immobilized on AuNPs of different size and concentration via the CALNN anchoring sequence. A relationship was established between the total surface area of the AuNPs (binding availability) and response (centroid shift). Moreover, a colorimetric assay for cTnI operating under optimized conditions (36 nm AuNPs) yielded a limit of detection of 0.2 ng/mL (8.4 pM) when tested in diluted serum samples with an assay time of 10 min. This encouraging result opens up for further development of AuNP assays in early diagnosis of cardiac injury.

KW - cardiac troponin I

KW - colorimetric sensing

KW - concentration of AuNPs

KW - peptide-functionalized gold nanoparticles

KW - size of AuNPs

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Peptide Functionalized Gold Nanoparticles with Optimized Particle Size and Concentration for Colorimetric Assay Development: Detection of Cardiac Troponin i

Abstract

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ASJC Scopus Subject Areas

Keywords

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