TY - JOUR
T1 - Phosphate regulation in Pseudomonas aeruginosa
T2 - Cloning of the alkaline phosphatase gene and identification of phoB- and phoR-like genes
AU - Filloux, A.
AU - Bally, M.
AU - Soscia, C.
AU - Murgier, M.
AU - Lazdunski, A.
PY - 1988/6
Y1 - 1988/6
N2 - In Pseudomonas aeruginosa, phosphate limitation results in the synthesis of several protein species. We report the cloning of the P. aeruginosa alkaline phosphatase structural gene, phoA, and we show that this gene is regulated normally in Escherichia coli. We have also identified and cloned two P. aeruginosa genes which can complement phoB and phoR mutations in E. coli. This suggests that a pho regulon system similar to that in E. coli may exist in P. aeruginosa, using at least two similar regulatory factors.
AB - In Pseudomonas aeruginosa, phosphate limitation results in the synthesis of several protein species. We report the cloning of the P. aeruginosa alkaline phosphatase structural gene, phoA, and we show that this gene is regulated normally in Escherichia coli. We have also identified and cloned two P. aeruginosa genes which can complement phoB and phoR mutations in E. coli. This suggests that a pho regulon system similar to that in E. coli may exist in P. aeruginosa, using at least two similar regulatory factors.
KW - Alkaline phosphatase
KW - Complementation
KW - Heterologous gene expression
KW - Phosphate regulation
KW - Pseudomonas aeruginosa
UR - http://www.scopus.com/inward/record.url?scp=0023924421&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023924421&partnerID=8YFLogxK
U2 - 10.1007/BF00330857
DO - 10.1007/BF00330857
M3 - Article
C2 - 3138529
AN - SCOPUS:0023924421
SN - 0026-8925
VL - 212
SP - 510
EP - 513
JO - Molecular Genetics and Genomics
JF - Molecular Genetics and Genomics
IS - 3
ER -