Photoacoustic imaging of lamina cribrosa microcapillaries in porcine eyes

Thanadet Chuangsuwanich, Mohesh Moothanchery, Alvan Tsz Chung Yan, Leopold Schmetterer, Michaël J.A. Girard, Manojit Pramanik*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

Due to the embedded nature of the lamina cribrosa (LC) microcapillary network, conventional imaging techniques have failed to obtain the high-resolution images needed to assess the perfusion state of the LC. In this study, both optical resolution (OR) and acoustic resolution (AR) photoacoustic microscopy (PAM) techniques were used to obtain static and dynamic information about LC perfusion in ex vivo porcine eyes. The OR-PAM system could resolve a perfused LC microcapillary network with a lateral resolution of 4.2 μm and also provided good depth information (33 μm axial resolution) to visualize through-thickness vascular variations. The AR-PAM system was capable of detecting time-dependent perfusion variations. This study represents the first step towards using an emerging imaging modality (PAM) to study the LC’s perfusion, which could be a basis for further investigation of the hemodynamic aspects of glaucomatous optic neuropathy.

Original languageEnglish
Pages (from-to)4865-4871
Number of pages7
JournalApplied Optics
Volume57
Issue number17
DOIs
Publication statusPublished - Jun 10 2018
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2018 Optical Society of America

ASJC Scopus Subject Areas

  • Atomic and Molecular Physics, and Optics
  • Engineering (miscellaneous)
  • Electrical and Electronic Engineering

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