Preparation and dynamic behavior of protein-polymer complexes formed with polymer-binding peptides

Samyukta Ravishankar; Seigo Suzuki; Toshiki Sawada; Sierin Lim; Takeshi Serizawa

doi:10.1246/bcsj.20200021

Preparation and dynamic behavior of protein-polymer complexes formed with polymer-binding peptides

Samyukta Ravishankar, Seigo Suzuki, Toshiki Sawada, Sierin Lim^*, Takeshi Serizawa^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

The development of protein-polymer complexes using protein cages is garnering attention as a strategy to overcome challenges that relate to immunogenicity and protease degradation. We propose the use of thermoresponsive polymers such as poly(N-isopropylacrylamide) (PNIPAM) to form ferritinpolymer complexes through non-covalent interactions. Ferritin is observed to be released from the complexes after 12 days incubation at 37 °C.

Original language	English
Pages (from-to)	790-793
Number of pages	4
Journal	Bulletin of the Chemical Society of Japan
Volume	93
Issue number	6
DOIs	https://doi.org/10.1246/bcsj.20200021
Publication status	Published - Jun 15 2020
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2021 The Chemical Society of Japan.

ASJC Scopus Subject Areas

General Chemistry

Keywords

Ferritin
Polymer-binding peptides
Protein-PNIPAM complexes

Access to Document

10.1246/bcsj.20200021

Cite this

@article{3f4d6f14508646878b982aff4ae60578,

title = "Preparation and dynamic behavior of protein-polymer complexes formed with polymer-binding peptides",

abstract = "The development of protein-polymer complexes using protein cages is garnering attention as a strategy to overcome challenges that relate to immunogenicity and protease degradation. We propose the use of thermoresponsive polymers such as poly(N-isopropylacrylamide) (PNIPAM) to form ferritinpolymer complexes through non-covalent interactions. Ferritin is observed to be released from the complexes after 12 days incubation at 37 °C.",

keywords = "Ferritin, Polymer-binding peptides, Protein-PNIPAM complexes",

author = "Samyukta Ravishankar and Seigo Suzuki and Toshiki Sawada and Sierin Lim and Takeshi Serizawa",

note = "Publisher Copyright: {\textcopyright} 2021 The Chemical Society of Japan.",

year = "2020",

month = jun,

day = "15",

doi = "10.1246/bcsj.20200021",

language = "English",

volume = "93",

pages = "790--793",

journal = "Bulletin of the Chemical Society of Japan",

issn = "0009-2673",

publisher = "Chemical Society of Japan",

number = "6",

}

TY - JOUR

T1 - Preparation and dynamic behavior of protein-polymer complexes formed with polymer-binding peptides

AU - Ravishankar, Samyukta

AU - Suzuki, Seigo

AU - Sawada, Toshiki

AU - Lim, Sierin

AU - Serizawa, Takeshi

PY - 2020/6/15

Y1 - 2020/6/15

N2 - The development of protein-polymer complexes using protein cages is garnering attention as a strategy to overcome challenges that relate to immunogenicity and protease degradation. We propose the use of thermoresponsive polymers such as poly(N-isopropylacrylamide) (PNIPAM) to form ferritinpolymer complexes through non-covalent interactions. Ferritin is observed to be released from the complexes after 12 days incubation at 37 °C.

AB - The development of protein-polymer complexes using protein cages is garnering attention as a strategy to overcome challenges that relate to immunogenicity and protease degradation. We propose the use of thermoresponsive polymers such as poly(N-isopropylacrylamide) (PNIPAM) to form ferritinpolymer complexes through non-covalent interactions. Ferritin is observed to be released from the complexes after 12 days incubation at 37 °C.

KW - Ferritin

KW - Polymer-binding peptides

KW - Protein-PNIPAM complexes

UR - http://www.scopus.com/inward/record.url?scp=85096545206&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85096545206&partnerID=8YFLogxK

U2 - 10.1246/bcsj.20200021

DO - 10.1246/bcsj.20200021

M3 - Article

AN - SCOPUS:85096545206

SN - 0009-2673

VL - 93

SP - 790

EP - 793

JO - Bulletin of the Chemical Society of Japan

JF - Bulletin of the Chemical Society of Japan

IS - 6

ER -

Preparation and dynamic behavior of protein-polymer complexes formed with polymer-binding peptides

Abstract

Bibliographical note

ASJC Scopus Subject Areas

Keywords

Access to Document

Other files and links

Fingerprint

Cite this