Presynaptic CaV2 calcium channel traffic requires CALF-1 and the α 2 subunit UNC-36

Yasunori Saheki; Cornelia I. Bargmann

doi:10.1038/nn.2383

Presynaptic CaV2 calcium channel traffic requires CALF-1 and the α 2 subunit UNC-36

Yasunori Saheki, Cornelia I. Bargmann

Rockefeller University

Research output: Contribution to journal › Article › peer-review

71 Citations (Scopus)

Abstract

Presynaptic voltage-gated calcium channels provide calcium for synaptic vesicle exocytosis. We show here that a green fluorescent protein-tagged α 1 subunit of the Caenorhabditis elegans CaV2 channel, UNC-2, is localized to presynaptic active zones of sensory and motor neurons. Synaptic localization of CaV2 requires the α 2 subunit UNC-36 and CALF-1 (Calcium Channel Localization Factor-1), a neuronal transmembrane protein that localizes to the endoplasmic reticulum. In calf-1 mutants, UNC-2 is retained in the endoplasmic reticulum, but other active-zone components and synaptic vesicles are delivered to synapses. Acute induction of calf-1 mobilizes preexisting UNC-2 for delivery to synapses, consistent with a direct trafficking role. The α 2 subunit UNC-36 is likewise required for exit of UNC-2 from endoplasmic reticulum but has additional functions. Genetic and cell biological interactions suggest that CALF-1 couples intracellular traffic to functional maturation of CaV2 presynaptic calcium channels.

Original language	English
Pages (from-to)	1257-1265
Number of pages	9
Journal	Nature Neuroscience
Volume	12
Issue number	10
DOIs	https://doi.org/10.1038/nn.2383
Publication status	Published - Oct 2009
Externally published	Yes

ASJC Scopus Subject Areas

General Neuroscience

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title = "Presynaptic CaV2 calcium channel traffic requires CALF-1 and the α 2 subunit UNC-36",

abstract = "Presynaptic voltage-gated calcium channels provide calcium for synaptic vesicle exocytosis. We show here that a green fluorescent protein-tagged α 1 subunit of the Caenorhabditis elegans CaV2 channel, UNC-2, is localized to presynaptic active zones of sensory and motor neurons. Synaptic localization of CaV2 requires the α 2 subunit UNC-36 and CALF-1 (Calcium Channel Localization Factor-1), a neuronal transmembrane protein that localizes to the endoplasmic reticulum. In calf-1 mutants, UNC-2 is retained in the endoplasmic reticulum, but other active-zone components and synaptic vesicles are delivered to synapses. Acute induction of calf-1 mobilizes preexisting UNC-2 for delivery to synapses, consistent with a direct trafficking role. The α 2 subunit UNC-36 is likewise required for exit of UNC-2 from endoplasmic reticulum but has additional functions. Genetic and cell biological interactions suggest that CALF-1 couples intracellular traffic to functional maturation of CaV2 presynaptic calcium channels.",

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Presynaptic CaV2 calcium channel traffic requires CALF-1 and the α 2 subunit UNC-36

Abstract

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