Profiling activity of cellular kinases in migrating T-cells

Chandra Sekhar Chirumamilla; Mobashar Hussain Urf Turabe Fazil; Claudina Perez-Novo; Savithri Rangarajan; Rik de Wijn; Padma Ramireddy; Navin Kumar Verma; Wim Vanden Berghe

doi:10.1007/978-1-4939-9036-8_13

Profiling activity of cellular kinases in migrating T-cells

Chandra Sekhar Chirumamilla, Mobashar Hussain Urf Turabe Fazil^*, Claudina Perez-Novo, Savithri Rangarajan, Rik de Wijn, Padma Ramireddy, Navin Kumar Verma, Wim Vanden Berghe

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter

40 Citations (Scopus)

Abstract

T-Lymphocyte kinases are important checkpoints that control T-cell motility by regulating a diverse range of signal transduction pathways. The distinct configuration of kinase events in T-cell could be used to fingerprint the status of T-cells. However, only small fraction human kinases have been characterized so far and little is known about the dynamics of the kinome in motile T-cells. Although several direct and indirect strategies exist to characterize cellular kinase activities, such as RNA interference, antibody arrays, enzyme kinetics, and mass spectrometry, this chapter focuses on an alternative multiplex phosphopeptide array-based methodology, which allows the kinome-wide identification of hyper-activated kinases involved in the regulation of T-cell migration.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	99-113
Number of pages	15
DOIs	https://doi.org/10.1007/978-1-4939-9036-8_13
Publication status	Published - 2019
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	1930
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© 2019, Springer Science+Business Media, LLC, part of Springer Nature.

ASJC Scopus Subject Areas

Molecular Biology
Genetics

Keywords

ICAM-1
Kinase
Kinome analysis
LFA-1
Peptide microarray
T-cell

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10.1007/978-1-4939-9036-8_13

Cite this

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AU - Rangarajan, Savithri

AU - de Wijn, Rik

AU - Ramireddy, Padma

AU - Verma, Navin Kumar

AU - Vanden Berghe, Wim

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Profiling activity of cellular kinases in migrating T-cells

Abstract

Publication series

Bibliographical note

ASJC Scopus Subject Areas

Keywords

Access to Document

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