Protein Cage-Stabilized Emulsions: Formulation and Characterization

Mridul Sarker; Samuel Watts; Stefan Salentinig; Sierin Lim

doi:10.1007/978-1-0716-3222-2_13

Protein Cage-Stabilized Emulsions: Formulation and Characterization

Mridul Sarker, Samuel Watts, Stefan Salentinig, Sierin Lim^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Citation (Scopus)

Abstract

The formulation of Pickering emulsions using protein cages is gaining interest for applications in molecular delivery. Despite the growing interest, methods to investigate the at the liquid-liquid interface are limited. This chapter describes standard methods to formulate and protocols to characterize protein cage-stabilized emulsions. The characterization methods are dynamic light scattering (DLS), intrinsic fluorescence spectroscopy (TF), circular dichroism (CD), and small angle X-ray scattering (SAXS). Combining these methods allows understanding of the protein cage nanostructure at the oil/water interface.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	219-239
Number of pages	21
DOIs	https://doi.org/10.1007/978-1-0716-3222-2_13
Publication status	Published - 2023
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	2671
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Publisher Copyright:
© 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

ASJC Scopus Subject Areas

Molecular Biology
Genetics

Keywords

Circular dichroism
DLS
Emulsion
Formulation
Intrinsic fluorescence
SAXS

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10.1007/978-1-0716-3222-2_13

Cite this

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abstract = "The formulation of Pickering emulsions using protein cages is gaining interest for applications in molecular delivery. Despite the growing interest, methods to investigate the at the liquid-liquid interface are limited. This chapter describes standard methods to formulate and protocols to characterize protein cage-stabilized emulsions. The characterization methods are dynamic light scattering (DLS), intrinsic fluorescence spectroscopy (TF), circular dichroism (CD), and small angle X-ray scattering (SAXS). Combining these methods allows understanding of the protein cage nanostructure at the oil/water interface.",

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T2 - Formulation and Characterization

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AU - Watts, Samuel

AU - Salentinig, Stefan

AU - Lim, Sierin

PY - 2023

Y1 - 2023

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AB - The formulation of Pickering emulsions using protein cages is gaining interest for applications in molecular delivery. Despite the growing interest, methods to investigate the at the liquid-liquid interface are limited. This chapter describes standard methods to formulate and protocols to characterize protein cage-stabilized emulsions. The characterization methods are dynamic light scattering (DLS), intrinsic fluorescence spectroscopy (TF), circular dichroism (CD), and small angle X-ray scattering (SAXS). Combining these methods allows understanding of the protein cage nanostructure at the oil/water interface.

KW - Circular dichroism

KW - DLS

KW - Emulsion

KW - Formulation

KW - Intrinsic fluorescence

KW - SAXS

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T3 - Methods in Molecular Biology

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BT - Methods in Molecular Biology

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ER -

Protein Cage-Stabilized Emulsions: Formulation and Characterization

Abstract

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Bibliographical note

ASJC Scopus Subject Areas

Keywords

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