Proteomic analysis of vascular smooth muscle cells with S- and R-Enantiomers of atenolol by iTRAQ and LC-MS/MS

Jianhua Zhang, Jiahua Shi, Benedict Jia Hong Lee, Liwei Chen, Kee Yang Tan, Xiaoling Tang, Jane Yi Lin Tan, Xiang Li, Huixing Feng, Wei Ning Chen

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

The term "proteome" was fi rst introduced by referring to the complete determination of proteins expressed in a given cell, tissue, or organism. In HPLC-based proteomics technique, the mixture of the cleaved peptides are bonded, separated sequentially on the multidimensional columns based on charge or hydrophobicity of the ionized analytes, and then eluted into the MS for identi fi cation. Among the developed stable isotope-based quanti fi cation methods, iTRAQ has recently gained popularity as its simple iTRAQ labeling procedures and up to eight labeled samples examined in a single experiment.

Original languageEnglish
Title of host publicationVascular Proteomics
Subtitle of host publicationMethods and Protocols
EditorsFernando Vivanco
Pages45-52
Number of pages8
DOIs
Publication statusPublished - 2013
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1000
ISSN (Print)1064-3745

ASJC Scopus Subject Areas

  • Molecular Biology
  • Genetics

Keywords

  • Atenolol
  • Cardiac adrenoreceptors
  • Chiral drug
  • ITRAQ
  • LC-MS/MS
  • Proteomics

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