Pseudomonas aeruginosa Psl exopolysaccharide interacts with the antimicrobial peptide LG21

Joyce Seow Fong Chin; Sheetal Sinha; Anjaiah Nalaparaju; Joey Kuok Hoong Yam; Zhiqiang Qin; Luyan Ma; Zhao Xun Liang; Lanyuan Lu; Surajit Bhattacharjya; Liang Yang

doi:10.3390/w9090681

Pseudomonas aeruginosa Psl exopolysaccharide interacts with the antimicrobial peptide LG21

Joyce Seow Fong Chin, Sheetal Sinha, Anjaiah Nalaparaju, Joey Kuok Hoong Yam, Zhiqiang Qin, Luyan Ma, Zhao Xun Liang, Lanyuan Lu, Surajit Bhattacharjya, Liang Yang^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Biofilm formation by opportunistic pathogens serves as one of the major causes of chronic and persistent infections. Bacterial cells in the biofilms are embedded in their self-generated protective extracellular polymeric substances (EPS), which include exopolysaccharides, large adhesin proteins and extracellular DNA. In this study, we identified an antimicrobial peptide (AMP) LG21 that is able to interact specifically with the Psl exopolysaccharide of Pseudomonas aeruginosa, thus it can be used as a diagnostic tool for P. aeruginosa biofilms. Molecular dynamics simulation analysis showed that residues numbered from 15 to 21 (WKRKRFG) in LG21 are involved in interacting with Psl. Our study indicates that host immune systems might detect and interact with microbial biofilms through AMPs. Engineering biofilm EPS-targeting AMPs might provide novel strategies for biofilm detection and treatment.

Original language	English
Article number	681
Journal	Water (Switzerland)
Volume	9
Issue number	9
DOIs	https://doi.org/10.3390/w9090681
Publication status	Published - Sept 16 2017
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2017 by the authors.

ASJC Scopus Subject Areas

Biochemistry
Geography, Planning and Development
Aquatic Science
Water Science and Technology

Keywords

Antimicrobial peptide (AMP)
Biofilm
EPS
Exopolysaccharide
Pseudomonas aeruginosa
Psl

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10.3390/w9090681

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title = "Pseudomonas aeruginosa Psl exopolysaccharide interacts with the antimicrobial peptide LG21",

abstract = "Biofilm formation by opportunistic pathogens serves as one of the major causes of chronic and persistent infections. Bacterial cells in the biofilms are embedded in their self-generated protective extracellular polymeric substances (EPS), which include exopolysaccharides, large adhesin proteins and extracellular DNA. In this study, we identified an antimicrobial peptide (AMP) LG21 that is able to interact specifically with the Psl exopolysaccharide of Pseudomonas aeruginosa, thus it can be used as a diagnostic tool for P. aeruginosa biofilms. Molecular dynamics simulation analysis showed that residues numbered from 15 to 21 (WKRKRFG) in LG21 are involved in interacting with Psl. Our study indicates that host immune systems might detect and interact with microbial biofilms through AMPs. Engineering biofilm EPS-targeting AMPs might provide novel strategies for biofilm detection and treatment.",

keywords = "Antimicrobial peptide (AMP), Biofilm, EPS, Exopolysaccharide, Pseudomonas aeruginosa, Psl",

author = "Chin, {Joyce Seow Fong} and Sheetal Sinha and Anjaiah Nalaparaju and Yam, {Joey Kuok Hoong} and Zhiqiang Qin and Luyan Ma and Liang, {Zhao Xun} and Lanyuan Lu and Surajit Bhattacharjya and Liang Yang",

note = "Publisher Copyright: {\textcopyright} 2017 by the authors.",

year = "2017",

month = sep,

day = "16",

doi = "10.3390/w9090681",

language = "English",

volume = "9",

journal = "Water (Switzerland)",

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T1 - Pseudomonas aeruginosa Psl exopolysaccharide interacts with the antimicrobial peptide LG21

AU - Chin, Joyce Seow Fong

AU - Sinha, Sheetal

AU - Nalaparaju, Anjaiah

AU - Yam, Joey Kuok Hoong

AU - Qin, Zhiqiang

AU - Ma, Luyan

AU - Liang, Zhao Xun

AU - Lu, Lanyuan

AU - Bhattacharjya, Surajit

AU - Yang, Liang

PY - 2017/9/16

Y1 - 2017/9/16

N2 - Biofilm formation by opportunistic pathogens serves as one of the major causes of chronic and persistent infections. Bacterial cells in the biofilms are embedded in their self-generated protective extracellular polymeric substances (EPS), which include exopolysaccharides, large adhesin proteins and extracellular DNA. In this study, we identified an antimicrobial peptide (AMP) LG21 that is able to interact specifically with the Psl exopolysaccharide of Pseudomonas aeruginosa, thus it can be used as a diagnostic tool for P. aeruginosa biofilms. Molecular dynamics simulation analysis showed that residues numbered from 15 to 21 (WKRKRFG) in LG21 are involved in interacting with Psl. Our study indicates that host immune systems might detect and interact with microbial biofilms through AMPs. Engineering biofilm EPS-targeting AMPs might provide novel strategies for biofilm detection and treatment.

AB - Biofilm formation by opportunistic pathogens serves as one of the major causes of chronic and persistent infections. Bacterial cells in the biofilms are embedded in their self-generated protective extracellular polymeric substances (EPS), which include exopolysaccharides, large adhesin proteins and extracellular DNA. In this study, we identified an antimicrobial peptide (AMP) LG21 that is able to interact specifically with the Psl exopolysaccharide of Pseudomonas aeruginosa, thus it can be used as a diagnostic tool for P. aeruginosa biofilms. Molecular dynamics simulation analysis showed that residues numbered from 15 to 21 (WKRKRFG) in LG21 are involved in interacting with Psl. Our study indicates that host immune systems might detect and interact with microbial biofilms through AMPs. Engineering biofilm EPS-targeting AMPs might provide novel strategies for biofilm detection and treatment.

KW - Antimicrobial peptide (AMP)

KW - Biofilm

KW - EPS

KW - Exopolysaccharide

KW - Pseudomonas aeruginosa

KW - Psl

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DO - 10.3390/w9090681

M3 - Article

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SN - 2073-4441

VL - 9

JO - Water (Switzerland)

JF - Water (Switzerland)

IS - 9

M1 - 681

ER -

Pseudomonas aeruginosa Psl exopolysaccharide interacts with the antimicrobial peptide LG21

Abstract

Bibliographical note

ASJC Scopus Subject Areas

Keywords

Access to Document

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