Rapid detection of Paenibacillus larvae from honey and hive samples with a novel nested PCR protocol

Federico M. Lauro; Matteo Favaretto; Loredana Covolo; Mario Rassu; Giulio Bertoloni

doi:10.1016/S0168-1605(02)00257-X

Rapid detection of Paenibacillus larvae from honey and hive samples with a novel nested PCR protocol

Federico M. Lauro, Matteo Favaretto, Loredana Covolo, Mario Rassu, Giulio Bertoloni^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

47 Citations (Scopus)

Abstract

The bacterial pathogen Paenibacillus larvae is the causative agent of American foulbrood disease in honeybees (Apis mellifera). A touchdown nested PCR protocol was developed to detect the presence of P. larvae spores directly in honey and hive samples. This approach allows early discovery of the bacteria even at concentrations below pathogenic levels, opening the door to new prophylactic approaches against American foulbrood and real-time epidemiological studies.

Original language	English
Pages (from-to)	195-201
Number of pages	7
Journal	International Journal of Food Microbiology
Volume	81
Issue number	3
DOIs	https://doi.org/10.1016/S0168-1605(02)00257-X
Publication status	Published - Mar 25 2003
Externally published	Yes

ASJC Scopus Subject Areas

Food Science
Microbiology

Keywords

American foulbrood
Apis mellifera
Honeybee
Paenibacillus larvae
PCR

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10.1016/S0168-1605(02)00257-X

Cite this

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title = "Rapid detection of Paenibacillus larvae from honey and hive samples with a novel nested PCR protocol",

abstract = "The bacterial pathogen Paenibacillus larvae is the causative agent of American foulbrood disease in honeybees (Apis mellifera). A touchdown nested PCR protocol was developed to detect the presence of P. larvae spores directly in honey and hive samples. This approach allows early discovery of the bacteria even at concentrations below pathogenic levels, opening the door to new prophylactic approaches against American foulbrood and real-time epidemiological studies.",

keywords = "American foulbrood, Apis mellifera, Honeybee, Paenibacillus larvae, PCR",

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AU - Lauro, Federico M.

AU - Favaretto, Matteo

AU - Covolo, Loredana

AU - Rassu, Mario

AU - Bertoloni, Giulio

PY - 2003/3/25

Y1 - 2003/3/25

N2 - The bacterial pathogen Paenibacillus larvae is the causative agent of American foulbrood disease in honeybees (Apis mellifera). A touchdown nested PCR protocol was developed to detect the presence of P. larvae spores directly in honey and hive samples. This approach allows early discovery of the bacteria even at concentrations below pathogenic levels, opening the door to new prophylactic approaches against American foulbrood and real-time epidemiological studies.

AB - The bacterial pathogen Paenibacillus larvae is the causative agent of American foulbrood disease in honeybees (Apis mellifera). A touchdown nested PCR protocol was developed to detect the presence of P. larvae spores directly in honey and hive samples. This approach allows early discovery of the bacteria even at concentrations below pathogenic levels, opening the door to new prophylactic approaches against American foulbrood and real-time epidemiological studies.

KW - American foulbrood

KW - Apis mellifera

KW - Honeybee

KW - Paenibacillus larvae

KW - PCR

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JO - International Journal of Food Microbiology

JF - International Journal of Food Microbiology

IS - 3

ER -

Rapid detection of Paenibacillus larvae from honey and hive samples with a novel nested PCR protocol

Abstract

ASJC Scopus Subject Areas

Keywords

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