Real-time impedance-based detection of LFA-1-stimulated T-cell transwell chemotaxis

Praseetha Prasannan; Navin Kumar Verma

doi:10.1007/978-1-4939-9036-8_7

Real-time impedance-based detection of LFA-1-stimulated T-cell transwell chemotaxis

Praseetha Prasannan^*, Navin Kumar Verma

^*Corresponding author for this work

Nanyang Technological University

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Citation (Scopus)

Abstract

The ability of activated T-lymphocytes to transmigrate toward certain chemokine is one of their characteristic functional properties. Here, we provide step-wise details about an in vitro technique to quantify the kinetics of chemotactic behavior of LFA-1-stimulated T-lymphocytes. The method described herein utilizes a noninvasive electrical impedance-based detection system to monitor T-cell chemotaxis in real-time.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	51-57
Number of pages	7
DOIs	https://doi.org/10.1007/978-1-4939-9036-8_7
Publication status	Published - 2019
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	1930
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© 2019, Springer Science+Business Media, LLC, part of Springer Nature.

ASJC Scopus Subject Areas

Molecular Biology
Genetics

Keywords

Chemoattractant
Chemotaxis
ICAM-1
LFA-1
SDF-1α
Transwell migration

Access to Document

10.1007/978-1-4939-9036-8_7

Cite this

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abstract = "The ability of activated T-lymphocytes to transmigrate toward certain chemokine is one of their characteristic functional properties. Here, we provide step-wise details about an in vitro technique to quantify the kinetics of chemotactic behavior of LFA-1-stimulated T-lymphocytes. The method described herein utilizes a noninvasive electrical impedance-based detection system to monitor T-cell chemotaxis in real-time.",

keywords = "Chemoattractant, Chemotaxis, ICAM-1, LFA-1, SDF-1α, Transwell migration",

author = "Praseetha Prasannan and Verma, {Navin Kumar}",

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language = "English",

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AU - Prasannan, Praseetha

AU - Verma, Navin Kumar

PY - 2019

Y1 - 2019

N2 - The ability of activated T-lymphocytes to transmigrate toward certain chemokine is one of their characteristic functional properties. Here, we provide step-wise details about an in vitro technique to quantify the kinetics of chemotactic behavior of LFA-1-stimulated T-lymphocytes. The method described herein utilizes a noninvasive electrical impedance-based detection system to monitor T-cell chemotaxis in real-time.

AB - The ability of activated T-lymphocytes to transmigrate toward certain chemokine is one of their characteristic functional properties. Here, we provide step-wise details about an in vitro technique to quantify the kinetics of chemotactic behavior of LFA-1-stimulated T-lymphocytes. The method described herein utilizes a noninvasive electrical impedance-based detection system to monitor T-cell chemotaxis in real-time.

KW - Chemoattractant

KW - Chemotaxis

KW - ICAM-1

KW - LFA-1

KW - SDF-1α

KW - Transwell migration

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AN - SCOPUS:85059499530

T3 - Methods in Molecular Biology

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BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Real-time impedance-based detection of LFA-1-stimulated T-cell transwell chemotaxis

Abstract

Publication series

Bibliographical note

ASJC Scopus Subject Areas

Keywords

Access to Document

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