Abstract
Mechanistic understanding of atherosclerosis is hampered by the lack of suitable in vitro human artery model. Current artery-on-chip models mostly focus on endothelial functions which are not suitable to study smooth muscle cells (SMC) in atherosclerosis. This work presents a novel microfluidic endothelial cells (EC)-SMC 3D co-culture platform to study both EC and SMC phenotypic changes. We first achieved SMC culture in quiescent state by optimizing ECM content. When stimulated with pro-inflammatory cytokines and oxidized low-density lipoprotein (oxLDL), we demonstrated SMC-oxLDL uptake and SMC migration as early atherogenic processes, which can be quantified at single cell level in our model.
| Original language | English |
|---|---|
| Title of host publication | MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 959-960 |
| Number of pages | 2 |
| ISBN (Electronic) | 9781733419017 |
| Publication status | Published - 2020 |
| Externally published | Yes |
| Event | 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 - Virtual, Online Duration: Oct 4 2020 → Oct 9 2020 |
Publication series
| Name | MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
|---|
Conference
| Conference | 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 |
|---|---|
| City | Virtual, Online |
| Period | 10/4/20 → 10/9/20 |
Bibliographical note
Publisher Copyright:© 2020 CBMS-0001
ASJC Scopus Subject Areas
- Chemical Engineering (miscellaneous)
- Bioengineering
- General Chemistry
- Control and Systems Engineering
Keywords
- Atherosclerosis
- Extracellular Matrix
- Microfluidics
- Organs-On-Chip
- Smooth Muscle Cells