RUNX3 interactome reveals novel centrosomal targeting of RUNX family of transcription factors

Linda Shyue Huey Chuang; Soak Kuan Lai; Maki Murata-Hori; Ayumi Yamada; Hoi Yeung Li; Jayantha Gunaratne; Yoshiaki Ito

doi:10.4161/cc.20278

RUNX3 interactome reveals novel centrosomal targeting of RUNX family of transcription factors

Linda Shyue Huey Chuang, Soak Kuan Lai, Maki Murata-Hori, Ayumi Yamada, Hoi Yeung Li, Jayantha Gunaratne, Yoshiaki Ito^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

RUNX family proteins are critical regulators of lineage differentiation during development. The high prevalence of RUNX mutation/epigenetic inactivation in human cancer indicates a causative role for dysfunctional RUNX in carcinogenesis. This is supported by well-documented evidence of functional interaction of RUNX with components of major oncogenic or tumor suppressive signaling pathways such as TGFβ and Wnt. Here, we explore the binding partners of RUNX3 proteins to further define the scope of RUNX3 function. Using a mass spectrometry-based approach, we found that RUNX3 binds to centrosomal protein rootletin. This led us to uncover the presence of RUNX proteins at the centrosome. Our findings suggest a potential function for RUNX3 during mitosis.

Original language	English
Pages (from-to)	1938-1947
Number of pages	10
Journal	Cell Cycle
Volume	11
Issue number	10
DOIs	https://doi.org/10.4161/cc.20278
Publication status	Published - May 15 2012
Externally published	Yes

ASJC Scopus Subject Areas

Molecular Biology
Developmental Biology
Cell Biology

Keywords

Centrosome
Mitotic spindle
Rootletin
RUNX
γ-tubulin

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.4161/cc.20278

Cite this

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title = "RUNX3 interactome reveals novel centrosomal targeting of RUNX family of transcription factors",

abstract = "RUNX family proteins are critical regulators of lineage differentiation during development. The high prevalence of RUNX mutation/epigenetic inactivation in human cancer indicates a causative role for dysfunctional RUNX in carcinogenesis. This is supported by well-documented evidence of functional interaction of RUNX with components of major oncogenic or tumor suppressive signaling pathways such as TGFβ and Wnt. Here, we explore the binding partners of RUNX3 proteins to further define the scope of RUNX3 function. Using a mass spectrometry-based approach, we found that RUNX3 binds to centrosomal protein rootletin. This led us to uncover the presence of RUNX proteins at the centrosome. Our findings suggest a potential function for RUNX3 during mitosis.",

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author = "Chuang, {Linda Shyue Huey} and Lai, {Soak Kuan} and Maki Murata-Hori and Ayumi Yamada and Li, {Hoi Yeung} and Jayantha Gunaratne and Yoshiaki Ito",

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doi = "10.4161/cc.20278",

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T1 - RUNX3 interactome reveals novel centrosomal targeting of RUNX family of transcription factors

AU - Chuang, Linda Shyue Huey

AU - Lai, Soak Kuan

AU - Murata-Hori, Maki

AU - Yamada, Ayumi

AU - Li, Hoi Yeung

AU - Gunaratne, Jayantha

AU - Ito, Yoshiaki

PY - 2012/5/15

Y1 - 2012/5/15

N2 - RUNX family proteins are critical regulators of lineage differentiation during development. The high prevalence of RUNX mutation/epigenetic inactivation in human cancer indicates a causative role for dysfunctional RUNX in carcinogenesis. This is supported by well-documented evidence of functional interaction of RUNX with components of major oncogenic or tumor suppressive signaling pathways such as TGFβ and Wnt. Here, we explore the binding partners of RUNX3 proteins to further define the scope of RUNX3 function. Using a mass spectrometry-based approach, we found that RUNX3 binds to centrosomal protein rootletin. This led us to uncover the presence of RUNX proteins at the centrosome. Our findings suggest a potential function for RUNX3 during mitosis.

AB - RUNX family proteins are critical regulators of lineage differentiation during development. The high prevalence of RUNX mutation/epigenetic inactivation in human cancer indicates a causative role for dysfunctional RUNX in carcinogenesis. This is supported by well-documented evidence of functional interaction of RUNX with components of major oncogenic or tumor suppressive signaling pathways such as TGFβ and Wnt. Here, we explore the binding partners of RUNX3 proteins to further define the scope of RUNX3 function. Using a mass spectrometry-based approach, we found that RUNX3 binds to centrosomal protein rootletin. This led us to uncover the presence of RUNX proteins at the centrosome. Our findings suggest a potential function for RUNX3 during mitosis.

KW - Centrosome

KW - Mitotic spindle

KW - Rootletin

KW - RUNX

KW - γ-tubulin

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RUNX3 interactome reveals novel centrosomal targeting of RUNX family of transcription factors

Abstract

ASJC Scopus Subject Areas

Keywords

UN SDGs

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