Simultaneous detection of Giardia lamblia and Cryptosporidium parvum (oo)cysts in soil using immunomagnetic separation and direct fluorescent antibody staining

Ezra Orlofsky; Osnat Gillor; Ann Melli; Woutrina Miller; Stefan Wuertz; Nirit Bernstein; Karen Shapiro

doi:10.1016/j.mimet.2013.07.019

Simultaneous detection of Giardia lamblia and Cryptosporidium parvum (oo)cysts in soil using immunomagnetic separation and direct fluorescent antibody staining

Ezra Orlofsky, Osnat Gillor, Ann Melli, Woutrina Miller, Stefan Wuertz, Nirit Bernstein, Karen Shapiro^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

An improved approach for simultaneous detection of Cryptosporidium parvum and Giardia lamblia (oo)cysts in soil is described. Recoveries>70% were obtained for concentrations>55 and 21 (oo)cysts g^-1 for C. parvum and G. lamblia, respectively. The limits of detection were determined to be<5 (oo)cysts g^-1 soil.

Original language	English
Pages (from-to)	375-377
Number of pages	3
Journal	Journal of Microbiological Methods
Volume	94
Issue number	3
DOIs	https://doi.org/10.1016/j.mimet.2013.07.019
Publication status	Published - Sept 2013
Externally published	Yes

ASJC Scopus Subject Areas

Microbiology
Molecular Biology
Microbiology (medical)

Keywords

Cryptosporidium parvum
Detection
Giardia lamblia
Immunomagnetic separation
Recovery
Soil contamination

Access to Document

10.1016/j.mimet.2013.07.019

Cite this

@article{36db7e961c3b48749dc756240ffed81a,

title = "Simultaneous detection of Giardia lamblia and Cryptosporidium parvum (oo)cysts in soil using immunomagnetic separation and direct fluorescent antibody staining",

abstract = "An improved approach for simultaneous detection of Cryptosporidium parvum and Giardia lamblia (oo)cysts in soil is described. Recoveries>70% were obtained for concentrations>55 and 21 (oo)cysts g-1 for C. parvum and G. lamblia, respectively. The limits of detection were determined to be<5 (oo)cysts g-1 soil.",

keywords = "Cryptosporidium parvum, Detection, Giardia lamblia, Immunomagnetic separation, Recovery, Soil contamination",

author = "Ezra Orlofsky and Osnat Gillor and Ann Melli and Woutrina Miller and Stefan Wuertz and Nirit Bernstein and Karen Shapiro",

year = "2013",

month = sep,

doi = "10.1016/j.mimet.2013.07.019",

language = "English",

volume = "94",

pages = "375--377",

journal = "Journal of Microbiological Methods",

issn = "0167-7012",

publisher = "Elsevier",

number = "3",

}

TY - JOUR

T1 - Simultaneous detection of Giardia lamblia and Cryptosporidium parvum (oo)cysts in soil using immunomagnetic separation and direct fluorescent antibody staining

AU - Orlofsky, Ezra

AU - Gillor, Osnat

AU - Melli, Ann

AU - Miller, Woutrina

AU - Wuertz, Stefan

AU - Bernstein, Nirit

AU - Shapiro, Karen

PY - 2013/9

Y1 - 2013/9

N2 - An improved approach for simultaneous detection of Cryptosporidium parvum and Giardia lamblia (oo)cysts in soil is described. Recoveries>70% were obtained for concentrations>55 and 21 (oo)cysts g-1 for C. parvum and G. lamblia, respectively. The limits of detection were determined to be<5 (oo)cysts g-1 soil.

AB - An improved approach for simultaneous detection of Cryptosporidium parvum and Giardia lamblia (oo)cysts in soil is described. Recoveries>70% were obtained for concentrations>55 and 21 (oo)cysts g-1 for C. parvum and G. lamblia, respectively. The limits of detection were determined to be<5 (oo)cysts g-1 soil.

KW - Cryptosporidium parvum

KW - Detection

KW - Giardia lamblia

KW - Immunomagnetic separation

KW - Recovery

KW - Soil contamination

UR - http://www.scopus.com/inward/record.url?scp=84883017398&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84883017398&partnerID=8YFLogxK

U2 - 10.1016/j.mimet.2013.07.019

DO - 10.1016/j.mimet.2013.07.019

M3 - Article

C2 - 23911570

AN - SCOPUS:84883017398

SN - 0167-7012

VL - 94

SP - 375

EP - 377

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 3

ER -

Simultaneous detection of Giardia lamblia and Cryptosporidium parvum (oo)cysts in soil using immunomagnetic separation and direct fluorescent antibody staining

Abstract

ASJC Scopus Subject Areas

Keywords

Access to Document

Other files and links

Fingerprint

Cite this