Simultaneous quantification of cells and nanomaterials by inductive-coupled plasma techniques

Alexandre Albanese; Kim M. Tsoi; Warren C.W. Chan

doi:10.1177/2211068212457039

Simultaneous quantification of cells and nanomaterials by inductive-coupled plasma techniques

Alexandre Albanese, Kim M. Tsoi, Warren C.W. Chan

University of Toronto

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

We demonstrate that endogenous cellular magnesium levels can be used as an accurate determinant of total cell number by inductively coupled plasma techniques, increasing the throughput and reproducibility of nanoparticle-uptake studies. Uptake of either gold nanoparticles or quantum dots did not affect intracellular concentration of Mg. To demonstrate this technique, we show the decreased uptake of nano-urchins in A549 cells compared with gold nanospheres.

Original language	English
Pages (from-to)	99-104
Number of pages	6
Journal	Journal of laboratory automation
Volume	18
Issue number	1
DOIs	https://doi.org/10.1177/2211068212457039
Publication status	Published - Feb 2013
Externally published	Yes

ASJC Scopus Subject Areas

Computer Science Applications
Medical Laboratory Technology

Keywords

Cell quantification
Cell uptake
Cellular elemental content
Gold nano-urchins
Gold nanoparticles
ICP-AES

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10.1177/2211068212457039

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abstract = "We demonstrate that endogenous cellular magnesium levels can be used as an accurate determinant of total cell number by inductively coupled plasma techniques, increasing the throughput and reproducibility of nanoparticle-uptake studies. Uptake of either gold nanoparticles or quantum dots did not affect intracellular concentration of Mg. To demonstrate this technique, we show the decreased uptake of nano-urchins in A549 cells compared with gold nanospheres.",

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AU - Tsoi, Kim M.

AU - Chan, Warren C.W.

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AB - We demonstrate that endogenous cellular magnesium levels can be used as an accurate determinant of total cell number by inductively coupled plasma techniques, increasing the throughput and reproducibility of nanoparticle-uptake studies. Uptake of either gold nanoparticles or quantum dots did not affect intracellular concentration of Mg. To demonstrate this technique, we show the decreased uptake of nano-urchins in A549 cells compared with gold nanospheres.

KW - Cell quantification

KW - Cell uptake

KW - Cellular elemental content

KW - Gold nano-urchins

KW - Gold nanoparticles

KW - ICP-AES

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Simultaneous quantification of cells and nanomaterials by inductive-coupled plasma techniques

Abstract

ASJC Scopus Subject Areas

Keywords

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