TY - JOUR
T1 - Spectral editing and pattern recognition methods applied to high-resolution magic-angle spinning 1H nuclear magnetic resonance spectroscopy of liver tissues
AU - Wang, Yulan
AU - Bollard, Mary E.
AU - Keun, Hector
AU - Antti, Henrik
AU - Beckonert, Olaf
AU - Ebbels, Timothy M.
AU - Lindon, John C.
AU - Holmes, Elaine
AU - Tang, Huiru
AU - Nicholson, Jeremy K.
PY - 2003/12/1
Y1 - 2003/12/1
N2 - Principal component analysis (PCA) has been applied to three nuclear magnetic resonance (NMR) spectral editing methods, namely, the Carr-Purcell-Meiboom-Gill spin-echo, diffusion editing, and skyline projection of a two-dimensional J-resolved spectrum, obtained from high-resolution magic-angle spinning NMR spectroscopy of liver tissues, to distinguish between control and hydrazine-treated rats. The effects of the toxin on rat liver biochemistry were directly observed and characterized by depleted levels of liver glycogen, choline, taurine, trimethylamine N-oxide, and glucose and by elevated levels of lipids and alanine. The highly unsaturated ω-3-type fatty acid was observed for the first time in hydrazine-treated rat liver. The contributions of the metabolites to the separation of control from dosed liver tissues varied depending on the type of spectral editing method used. We have shown that subtle changes in the metabolic profiles can be selectively amplified using a metabonomics approach based on the different NMR spectral editing techniques in conjunction with PCA.
AB - Principal component analysis (PCA) has been applied to three nuclear magnetic resonance (NMR) spectral editing methods, namely, the Carr-Purcell-Meiboom-Gill spin-echo, diffusion editing, and skyline projection of a two-dimensional J-resolved spectrum, obtained from high-resolution magic-angle spinning NMR spectroscopy of liver tissues, to distinguish between control and hydrazine-treated rats. The effects of the toxin on rat liver biochemistry were directly observed and characterized by depleted levels of liver glycogen, choline, taurine, trimethylamine N-oxide, and glucose and by elevated levels of lipids and alanine. The highly unsaturated ω-3-type fatty acid was observed for the first time in hydrazine-treated rat liver. The contributions of the metabolites to the separation of control from dosed liver tissues varied depending on the type of spectral editing method used. We have shown that subtle changes in the metabolic profiles can be selectively amplified using a metabonomics approach based on the different NMR spectral editing techniques in conjunction with PCA.
KW - HRMAS H NMR spectroscopy
KW - Hydrazine
KW - Liver tissue
KW - Metabonomics
KW - NMR spectral editing
KW - Principal components analysis
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U2 - 10.1016/j.ab.2003.07.026
DO - 10.1016/j.ab.2003.07.026
M3 - Article
C2 - 14622955
AN - SCOPUS:0242690435
SN - 0003-2697
VL - 323
SP - 26
EP - 32
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 1
ER -