Subcomplexes from the Xcp secretion system of Pseudomonas aeruginosa

Viviane Robert; Alain Filloux; Gérard P.F. Michel

doi:10.1016/j.femsle.2005.08.029

Subcomplexes from the Xcp secretion system of Pseudomonas aeruginosa

Viviane Robert, Alain Filloux, Gérard P.F. Michel^*

^*Corresponding author for this work

CNRS UPR 9027-IBSM

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

In Gram-negative bacteria, most of the sec-dependent exoproteins are secreted via the type II secretion system (T2SS or secreton). In Pseudomonas aeruginosa, T2SS consists of 12 Xcp proteins (XcpA and XcpP to XcpZ) organized as a multiproteic complex within the envelope. In this study, by a co-purification approach using a His-tagged XcpZ as a bait, XcpY and XcpZ were found associated together to constitute the most stable functional unit so far isolated from the P. aeruginosa secreton. This subcomplex was also found to interact with XcpR and XcpS to form a XcpRSYZ complex which was isolated under native conditions. Another component, XcpP was not found to be associated to the complex but the results suggest that it can transiently interact with the XcpYZ subcomplex in vivo.

Original language	English
Pages (from-to)	43-50
Number of pages	8
Journal	FEMS Microbiology Letters
Volume	252
Issue number	1
DOIs	https://doi.org/10.1016/j.femsle.2005.08.029
Publication status	Published - Nov 1 2005
Externally published	Yes

ASJC Scopus Subject Areas

Microbiology
Molecular Biology
Genetics

Keywords

Protein secretion
Protein-protein interactions
Pseudomonas aeruginosa
Secreton assembly
Type II secretion system
Xcp

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10.1016/j.femsle.2005.08.029

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title = "Subcomplexes from the Xcp secretion system of Pseudomonas aeruginosa",

abstract = "In Gram-negative bacteria, most of the sec-dependent exoproteins are secreted via the type II secretion system (T2SS or secreton). In Pseudomonas aeruginosa, T2SS consists of 12 Xcp proteins (XcpA and XcpP to XcpZ) organized as a multiproteic complex within the envelope. In this study, by a co-purification approach using a His-tagged XcpZ as a bait, XcpY and XcpZ were found associated together to constitute the most stable functional unit so far isolated from the P. aeruginosa secreton. This subcomplex was also found to interact with XcpR and XcpS to form a XcpRSYZ complex which was isolated under native conditions. Another component, XcpP was not found to be associated to the complex but the results suggest that it can transiently interact with the XcpYZ subcomplex in vivo.",

keywords = "Protein secretion, Protein-protein interactions, Pseudomonas aeruginosa, Secreton assembly, Type II secretion system, Xcp",

author = "Viviane Robert and Alain Filloux and Michel, \{G{\'e}rard P.F.\}",

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doi = "10.1016/j.femsle.2005.08.029",

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AU - Robert, Viviane

AU - Filloux, Alain

AU - Michel, Gérard P.F.

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N2 - In Gram-negative bacteria, most of the sec-dependent exoproteins are secreted via the type II secretion system (T2SS or secreton). In Pseudomonas aeruginosa, T2SS consists of 12 Xcp proteins (XcpA and XcpP to XcpZ) organized as a multiproteic complex within the envelope. In this study, by a co-purification approach using a His-tagged XcpZ as a bait, XcpY and XcpZ were found associated together to constitute the most stable functional unit so far isolated from the P. aeruginosa secreton. This subcomplex was also found to interact with XcpR and XcpS to form a XcpRSYZ complex which was isolated under native conditions. Another component, XcpP was not found to be associated to the complex but the results suggest that it can transiently interact with the XcpYZ subcomplex in vivo.

AB - In Gram-negative bacteria, most of the sec-dependent exoproteins are secreted via the type II secretion system (T2SS or secreton). In Pseudomonas aeruginosa, T2SS consists of 12 Xcp proteins (XcpA and XcpP to XcpZ) organized as a multiproteic complex within the envelope. In this study, by a co-purification approach using a His-tagged XcpZ as a bait, XcpY and XcpZ were found associated together to constitute the most stable functional unit so far isolated from the P. aeruginosa secreton. This subcomplex was also found to interact with XcpR and XcpS to form a XcpRSYZ complex which was isolated under native conditions. Another component, XcpP was not found to be associated to the complex but the results suggest that it can transiently interact with the XcpYZ subcomplex in vivo.

KW - Protein secretion

KW - Protein-protein interactions

KW - Pseudomonas aeruginosa

KW - Secreton assembly

KW - Type II secretion system

KW - Xcp

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Subcomplexes from the Xcp secretion system of Pseudomonas aeruginosa

Abstract

ASJC Scopus Subject Areas

Keywords

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