Targeted gene silencing in malignant hematolymphoid cells using gapmer

Atish Kizhakeyil; Mobashar Hussain Urf Turabe Fazil; Navin Kumar Verma

doi:10.1007/978-1-0716-0771-8_15

Targeted gene silencing in malignant hematolymphoid cells using gapmer

Atish Kizhakeyil^*, Mobashar Hussain Urf Turabe Fazil, Navin Kumar Verma

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Delivery of conventional antisense oligonucleotides or small interfering RNA (siRNA) molecules into hematolymphoid cells for targeted gene silencing has been proven to be difficult. Here, we describe a simple protocol to knockdown specific gene(s) in malignant hematolymphoid cells using “GapmeR.” This protocol could be applicable to a wide range of cell-types and thus solves an important problem for researchers working with cell lines or primary cells derived from patients with hematolymphoid malignancies.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	209-219
Number of pages	11
DOIs	https://doi.org/10.1007/978-1-0716-0771-8_15
Publication status	Published - 2020
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	2176
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2020.

ASJC Scopus Subject Areas

Molecular Biology
Genetics

Keywords

GapmeR
Gene knockdown
HuT78
Lymphoma cells
RNAi
STAT3

Access to Document

10.1007/978-1-0716-0771-8_15

Cite this

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abstract = "Delivery of conventional antisense oligonucleotides or small interfering RNA (siRNA) molecules into hematolymphoid cells for targeted gene silencing has been proven to be difficult. Here, we describe a simple protocol to knockdown specific gene(s) in malignant hematolymphoid cells using “GapmeR.” This protocol could be applicable to a wide range of cell-types and thus solves an important problem for researchers working with cell lines or primary cells derived from patients with hematolymphoid malignancies.",

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AU - Fazil, Mobashar Hussain Urf Turabe

AU - Verma, Navin Kumar

N1 - Publisher Copyright: © Springer Science+Business Media, LLC, part of Springer Nature 2020.

PY - 2020

Y1 - 2020

N2 - Delivery of conventional antisense oligonucleotides or small interfering RNA (siRNA) molecules into hematolymphoid cells for targeted gene silencing has been proven to be difficult. Here, we describe a simple protocol to knockdown specific gene(s) in malignant hematolymphoid cells using “GapmeR.” This protocol could be applicable to a wide range of cell-types and thus solves an important problem for researchers working with cell lines or primary cells derived from patients with hematolymphoid malignancies.

AB - Delivery of conventional antisense oligonucleotides or small interfering RNA (siRNA) molecules into hematolymphoid cells for targeted gene silencing has been proven to be difficult. Here, we describe a simple protocol to knockdown specific gene(s) in malignant hematolymphoid cells using “GapmeR.” This protocol could be applicable to a wide range of cell-types and thus solves an important problem for researchers working with cell lines or primary cells derived from patients with hematolymphoid malignancies.

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KW - HuT78

KW - Lymphoma cells

KW - RNAi

KW - STAT3

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ER -

Targeted gene silencing in malignant hematolymphoid cells using gapmer

Abstract

Publication series

Bibliographical note

ASJC Scopus Subject Areas

Keywords

Access to Document

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