The development of direct multicolour fluorescence cross-correlation spectroscopy: Towards a new tool for tracking complex biomolecular events in real-time

Holly M. Wobma; Megan L. Blades; Ekaterina Grekova; Dylan L. McGuire; Kun Chen; Warren C.W. Chan; David T. Cramb

doi:10.1039/c2cp23278b

The development of direct multicolour fluorescence cross-correlation spectroscopy: Towards a new tool for tracking complex biomolecular events in real-time

Holly M. Wobma, Megan L. Blades, Ekaterina Grekova, Dylan L. McGuire, Kun Chen, Warren C.W. Chan, David T. Cramb^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Direct three-colour fluorescence cross-correlation spectroscopy can reveal interactions between three fluorescently labelled biomolecules, giving insight toward the complex events that constitute signal transduction pathways. Here we provide the optical and theoretical basis for this technology and demonstrate its ability to detect specific biological associations between nanoparticle-labelled DNA molecules.

Original language	English
Pages (from-to)	3290-3294
Number of pages	5
Journal	Physical Chemistry Chemical Physics
Volume	14
Issue number	10
DOIs	https://doi.org/10.1039/c2cp23278b
Publication status	Published - Mar 14 2012
Externally published	Yes

ASJC Scopus Subject Areas

General Physics and Astronomy
Physical and Theoretical Chemistry

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10.1039/c2cp23278b

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title = "The development of direct multicolour fluorescence cross-correlation spectroscopy: Towards a new tool for tracking complex biomolecular events in real-time",

abstract = "Direct three-colour fluorescence cross-correlation spectroscopy can reveal interactions between three fluorescently labelled biomolecules, giving insight toward the complex events that constitute signal transduction pathways. Here we provide the optical and theoretical basis for this technology and demonstrate its ability to detect specific biological associations between nanoparticle-labelled DNA molecules.",

author = "Wobma, {Holly M.} and Blades, {Megan L.} and Ekaterina Grekova and McGuire, {Dylan L.} and Kun Chen and Chan, {Warren C.W.} and Cramb, {David T.}",

year = "2012",

month = mar,

day = "14",

doi = "10.1039/c2cp23278b",

language = "English",

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journal = "Physical Chemistry Chemical Physics",

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The development of direct multicolour fluorescence cross-correlation spectroscopy: Towards a new tool for tracking complex biomolecular events in real-time. / Wobma, Holly M.; Blades, Megan L.; Grekova, Ekaterina et al.
In: Physical Chemistry Chemical Physics, Vol. 14, No. 10, 14.03.2012, p. 3290-3294.

Research output: Contribution to journal › Article › peer-review

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T1 - The development of direct multicolour fluorescence cross-correlation spectroscopy

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AU - Wobma, Holly M.

AU - Blades, Megan L.

AU - Grekova, Ekaterina

AU - McGuire, Dylan L.

AU - Chen, Kun

AU - Chan, Warren C.W.

AU - Cramb, David T.

PY - 2012/3/14

Y1 - 2012/3/14

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AB - Direct three-colour fluorescence cross-correlation spectroscopy can reveal interactions between three fluorescently labelled biomolecules, giving insight toward the complex events that constitute signal transduction pathways. Here we provide the optical and theoretical basis for this technology and demonstrate its ability to detect specific biological associations between nanoparticle-labelled DNA molecules.

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The development of direct multicolour fluorescence cross-correlation spectroscopy: Towards a new tool for tracking complex biomolecular events in real-time

Abstract

ASJC Scopus Subject Areas

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