The Effects of Nanofiber Topography on Astrocyte Behavior and Gene Silencing Efficiency

Haoqing Cao; Guillaume Marcy; Eyleen Lay Keow Goh; Feng Wang; Jun Wang; Sing Yian Chew

doi:10.1002/mabi.201100436

The Effects of Nanofiber Topography on Astrocyte Behavior and Gene Silencing Efficiency

Haoqing Cao, Guillaume Marcy, Eyleen Lay Keow Goh, Feng Wang, Jun Wang, Sing Yian Chew^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

Astrocyte-nanofiber interactions are studied by culturing primary rat cortical astrocytes on poly[caprolactone-co-(ethyl ethylene phosphate)] electrospun nanofibers and solvent-cast films (two-dimensional control). The results indicate that nanofiber topography significantly suppresses astrocyte proliferation and enhances apoptosis, without altering cellular activation as compared to films. Moreover, nanofiber topography enhances gene-silencing efficiency in astrocytes. The results suggest that nanofibers may serve as potential substrates for nerve regeneration by suppressing astrocyte growth and may further facilitate the use of gene-silencing to enhance CNS regeneration.

Original language	English
Pages (from-to)	666-674
Number of pages	9
Journal	Macromolecular Bioscience
Volume	12
Issue number	5
DOIs	https://doi.org/10.1002/mabi.201100436
Publication status	Published - May 2012
Externally published	Yes

ASJC Scopus Subject Areas

Biotechnology
Bioengineering
Biomaterials
Polymers and Plastics
Materials Chemistry

Keywords

Electrospinning
Fibers
Films
Neural tissue engineering
SiRNA

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10.1002/mabi.201100436

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title = "The Effects of Nanofiber Topography on Astrocyte Behavior and Gene Silencing Efficiency",

abstract = "Astrocyte-nanofiber interactions are studied by culturing primary rat cortical astrocytes on poly[caprolactone-co-(ethyl ethylene phosphate)] electrospun nanofibers and solvent-cast films (two-dimensional control). The results indicate that nanofiber topography significantly suppresses astrocyte proliferation and enhances apoptosis, without altering cellular activation as compared to films. Moreover, nanofiber topography enhances gene-silencing efficiency in astrocytes. The results suggest that nanofibers may serve as potential substrates for nerve regeneration by suppressing astrocyte growth and may further facilitate the use of gene-silencing to enhance CNS regeneration.",

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T1 - The Effects of Nanofiber Topography on Astrocyte Behavior and Gene Silencing Efficiency

AU - Cao, Haoqing

AU - Marcy, Guillaume

AU - Goh, Eyleen Lay Keow

AU - Wang, Feng

AU - Wang, Jun

AU - Chew, Sing Yian

PY - 2012/5

Y1 - 2012/5

N2 - Astrocyte-nanofiber interactions are studied by culturing primary rat cortical astrocytes on poly[caprolactone-co-(ethyl ethylene phosphate)] electrospun nanofibers and solvent-cast films (two-dimensional control). The results indicate that nanofiber topography significantly suppresses astrocyte proliferation and enhances apoptosis, without altering cellular activation as compared to films. Moreover, nanofiber topography enhances gene-silencing efficiency in astrocytes. The results suggest that nanofibers may serve as potential substrates for nerve regeneration by suppressing astrocyte growth and may further facilitate the use of gene-silencing to enhance CNS regeneration.

AB - Astrocyte-nanofiber interactions are studied by culturing primary rat cortical astrocytes on poly[caprolactone-co-(ethyl ethylene phosphate)] electrospun nanofibers and solvent-cast films (two-dimensional control). The results indicate that nanofiber topography significantly suppresses astrocyte proliferation and enhances apoptosis, without altering cellular activation as compared to films. Moreover, nanofiber topography enhances gene-silencing efficiency in astrocytes. The results suggest that nanofibers may serve as potential substrates for nerve regeneration by suppressing astrocyte growth and may further facilitate the use of gene-silencing to enhance CNS regeneration.

KW - Electrospinning

KW - Fibers

KW - Films

KW - Neural tissue engineering

KW - SiRNA

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JO - Macromolecular Bioscience

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The Effects of Nanofiber Topography on Astrocyte Behavior and Gene Silencing Efficiency

Abstract

ASJC Scopus Subject Areas

Keywords

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