The influence of microbial metabolites on human intestinal epithelial cells and macrophages in vitro

Marleen H.M.C. Van Nuenen; Rianne A.F. De Ligt; Robert P. Doornbos; Janneke C.J. Van Der Woude; Ernst J. Kuipers; Koen Venema

doi:10.1016/j.femsim.2005.03.010

The influence of microbial metabolites on human intestinal epithelial cells and macrophages in vitro

Marleen H.M.C. Van Nuenen^*, Rianne A.F. De Ligt, Robert P. Doornbos, Janneke C.J. Van Der Woude, Ernst J. Kuipers, Koen Venema

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

46 Citations (Scopus)

Abstract

Microbial metabolites may influence the metabolic integrity of intestinal epithelial cells and induce mucosal immune responses. Therefore, we investigated the effects of the microbial metabolites butyrate, iso-valerate, and ammonium on Caco-2 cells and macrophages. Barrier functioning was determined by measuring transepithelial electrical resistance and basolateral recoveries of metabolites. The barrier function of Caco-2 cells remained intact after exposures. Basolateral recoveries ranged from 6.2% to 15.2%. Tumour necrosis factor-α and interleukin-10 were measured to determine immune reactions. The Caco-2 cells did not secrete both cytokines. Physiological concentrations of butyrate and iso-valerate stimulated the secretion of tumour necrosis factor-α and suppressed the secretion of interleukin-10 by macrophages that are not protected by an epithelial barrier. In contrast, ammonium concentrations as high as those produced by microbiotas of IBD patients suppressed the release of both cytokines when the barrier function is impaired.

Original language	English
Pages (from-to)	183-189
Number of pages	7
Journal	FEMS Immunology and Medical Microbiology
Volume	45
Issue number	2
DOIs	https://doi.org/10.1016/j.femsim.2005.03.010
Publication status	Published - Aug 1 2005
Externally published	Yes

ASJC Scopus Subject Areas

Immunology and Allergy
Microbiology
Immunology
Microbiology (medical)
Infectious Diseases

Keywords

Interleukin-10
Intestinal epithelial cells
Macrophages
Microbial metabolites
Tumour necrosis factor-α

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.femsim.2005.03.010

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title = "The influence of microbial metabolites on human intestinal epithelial cells and macrophages in vitro",

abstract = "Microbial metabolites may influence the metabolic integrity of intestinal epithelial cells and induce mucosal immune responses. Therefore, we investigated the effects of the microbial metabolites butyrate, iso-valerate, and ammonium on Caco-2 cells and macrophages. Barrier functioning was determined by measuring transepithelial electrical resistance and basolateral recoveries of metabolites. The barrier function of Caco-2 cells remained intact after exposures. Basolateral recoveries ranged from 6.2\% to 15.2\%. Tumour necrosis factor-α and interleukin-10 were measured to determine immune reactions. The Caco-2 cells did not secrete both cytokines. Physiological concentrations of butyrate and iso-valerate stimulated the secretion of tumour necrosis factor-α and suppressed the secretion of interleukin-10 by macrophages that are not protected by an epithelial barrier. In contrast, ammonium concentrations as high as those produced by microbiotas of IBD patients suppressed the release of both cytokines when the barrier function is impaired.",

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doi = "10.1016/j.femsim.2005.03.010",

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AU - Van Nuenen, Marleen H.M.C.

AU - De Ligt, Rianne A.F.

AU - Doornbos, Robert P.

AU - Van Der Woude, Janneke C.J.

AU - Kuipers, Ernst J.

AU - Venema, Koen

PY - 2005/8/1

Y1 - 2005/8/1

N2 - Microbial metabolites may influence the metabolic integrity of intestinal epithelial cells and induce mucosal immune responses. Therefore, we investigated the effects of the microbial metabolites butyrate, iso-valerate, and ammonium on Caco-2 cells and macrophages. Barrier functioning was determined by measuring transepithelial electrical resistance and basolateral recoveries of metabolites. The barrier function of Caco-2 cells remained intact after exposures. Basolateral recoveries ranged from 6.2% to 15.2%. Tumour necrosis factor-α and interleukin-10 were measured to determine immune reactions. The Caco-2 cells did not secrete both cytokines. Physiological concentrations of butyrate and iso-valerate stimulated the secretion of tumour necrosis factor-α and suppressed the secretion of interleukin-10 by macrophages that are not protected by an epithelial barrier. In contrast, ammonium concentrations as high as those produced by microbiotas of IBD patients suppressed the release of both cytokines when the barrier function is impaired.

AB - Microbial metabolites may influence the metabolic integrity of intestinal epithelial cells and induce mucosal immune responses. Therefore, we investigated the effects of the microbial metabolites butyrate, iso-valerate, and ammonium on Caco-2 cells and macrophages. Barrier functioning was determined by measuring transepithelial electrical resistance and basolateral recoveries of metabolites. The barrier function of Caco-2 cells remained intact after exposures. Basolateral recoveries ranged from 6.2% to 15.2%. Tumour necrosis factor-α and interleukin-10 were measured to determine immune reactions. The Caco-2 cells did not secrete both cytokines. Physiological concentrations of butyrate and iso-valerate stimulated the secretion of tumour necrosis factor-α and suppressed the secretion of interleukin-10 by macrophages that are not protected by an epithelial barrier. In contrast, ammonium concentrations as high as those produced by microbiotas of IBD patients suppressed the release of both cytokines when the barrier function is impaired.

KW - Interleukin-10

KW - Intestinal epithelial cells

KW - Macrophages

KW - Microbial metabolites

KW - Tumour necrosis factor-α

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The influence of microbial metabolites on human intestinal epithelial cells and macrophages in vitro

Abstract

ASJC Scopus Subject Areas

Keywords

UN SDGs

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