The microbiome of settling dust from childcare centres in Singapore

Akira Uchida; Irvan Luhung; Dana Miller; Sandra Kolundzija; Cassie E. Heinle; Kenny Lau; Premkrishnan B.N. Vasantha; Gayatri Sankaran; Ron Tan; Stephan C. Schuster

The microbiome of settling dust from childcare centres in Singapore

Akira Uchida, Irvan Luhung, Dana Miller, Sandra Kolundzija, Cassie E. Heinle, Kenny Lau, Premkrishnan B.N. Vasantha, Gayatri Sankaran, Ron Tan, Stephan C. Schuster^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

This study reveals biomass concentration and microbiome detected from settling dust samples collected from selected childcare centres (indoor and outdoor) in tropical climate with three ventilation types: centralized, split unit and natural ventilation. Dusts were collected by means of passive sampling by placing petri dishes at 2-meter height over 4 weeks. DNA was then extracted and analysed by fluorometry and metagenomic sequencing. The results indicated that there is no significant difference in DNA yield among different ventilation types and that a high variability was observed from petri dishes of the same site. The metagenomic analysis suggests that the indoor dust from the childcare centre with centralized air-con was the most different from outdoor microbiome, whereas the indoor dust from the naturally ventilated centres showed the most similarity to outdoor microbiome.

Original language	English
Title of host publication	15th Conference of the International Society of Indoor Air Quality and Climate, INDOOR AIR 2018
Publisher	International Society of Indoor Air Quality and Climate
ISBN (Electronic)	9781713826514
Publication status	Published - 2018
Externally published	Yes
Event	15th Conference of the International Society of Indoor Air Quality and Climate, INDOOR AIR 2018 - Philadelphia, United States Duration: Jul 22 2018 → Jul 27 2018

Publication series

Name	15th Conference of the International Society of Indoor Air Quality and Climate, INDOOR AIR 2018

Conference

Conference	15th Conference of the International Society of Indoor Air Quality and Climate, INDOOR AIR 2018
Country/Territory	United States
City	Philadelphia
Period	7/22/18 → 7/27/18

Bibliographical note

Publisher Copyright:
© 2018 15th Conference of the International Society of Indoor Air Quality and Climate, INDOOR AIR 2018. All rights reserved.

ASJC Scopus Subject Areas

Pollution

Keywords

Air conditioning system
Microbiome
Passive dust collection
Tropical climate bioaerosols

Cite this

Uchida, A., Luhung, I., Miller, D., Kolundzija, S., Heinle, C. E., Lau, K., Vasantha, P. B. N., Sankaran, G., Tan, R., & Schuster, S. C. (2018). The microbiome of settling dust from childcare centres in Singapore. In 15th Conference of the International Society of Indoor Air Quality and Climate, INDOOR AIR 2018 (15th Conference of the International Society of Indoor Air Quality and Climate, INDOOR AIR 2018). International Society of Indoor Air Quality and Climate.

Uchida, Akira ; Luhung, Irvan ; Miller, Dana et al. / The microbiome of settling dust from childcare centres in Singapore. 15th Conference of the International Society of Indoor Air Quality and Climate, INDOOR AIR 2018. International Society of Indoor Air Quality and Climate, 2018. (15th Conference of the International Society of Indoor Air Quality and Climate, INDOOR AIR 2018).

@inproceedings{cef4926cfb3344a4b938726672ee2d26,

title = "The microbiome of settling dust from childcare centres in Singapore",

abstract = "This study reveals biomass concentration and microbiome detected from settling dust samples collected from selected childcare centres (indoor and outdoor) in tropical climate with three ventilation types: centralized, split unit and natural ventilation. Dusts were collected by means of passive sampling by placing petri dishes at 2-meter height over 4 weeks. DNA was then extracted and analysed by fluorometry and metagenomic sequencing. The results indicated that there is no significant difference in DNA yield among different ventilation types and that a high variability was observed from petri dishes of the same site. The metagenomic analysis suggests that the indoor dust from the childcare centre with centralized air-con was the most different from outdoor microbiome, whereas the indoor dust from the naturally ventilated centres showed the most similarity to outdoor microbiome.",

keywords = "Air conditioning system, Microbiome, Passive dust collection, Tropical climate bioaerosols",

author = "Akira Uchida and Irvan Luhung and Dana Miller and Sandra Kolundzija and Heinle, {Cassie E.} and Kenny Lau and Vasantha, {Premkrishnan B.N.} and Gayatri Sankaran and Ron Tan and Schuster, {Stephan C.}",

note = "Publisher Copyright: {\textcopyright} 2018 15th Conference of the International Society of Indoor Air Quality and Climate, INDOOR AIR 2018. All rights reserved.; 15th Conference of the International Society of Indoor Air Quality and Climate, INDOOR AIR 2018 ; Conference date: 22-07-2018 Through 27-07-2018",

year = "2018",

language = "English",

series = "15th Conference of the International Society of Indoor Air Quality and Climate, INDOOR AIR 2018",

publisher = "International Society of Indoor Air Quality and Climate",

booktitle = "15th Conference of the International Society of Indoor Air Quality and Climate, INDOOR AIR 2018",

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Uchida, A, Luhung, I, Miller, D, Kolundzija, S, Heinle, CE, Lau, K, Vasantha, PBN, Sankaran, G, Tan, R & Schuster, SC 2018, The microbiome of settling dust from childcare centres in Singapore. in 15th Conference of the International Society of Indoor Air Quality and Climate, INDOOR AIR 2018. 15th Conference of the International Society of Indoor Air Quality and Climate, INDOOR AIR 2018, International Society of Indoor Air Quality and Climate, 15th Conference of the International Society of Indoor Air Quality and Climate, INDOOR AIR 2018, Philadelphia, United States, 7/22/18.

The microbiome of settling dust from childcare centres in Singapore. / Uchida, Akira; Luhung, Irvan; Miller, Dana et al.
15th Conference of the International Society of Indoor Air Quality and Climate, INDOOR AIR 2018. International Society of Indoor Air Quality and Climate, 2018. (15th Conference of the International Society of Indoor Air Quality and Climate, INDOOR AIR 2018).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - The microbiome of settling dust from childcare centres in Singapore

AU - Uchida, Akira

AU - Luhung, Irvan

AU - Miller, Dana

AU - Kolundzija, Sandra

AU - Heinle, Cassie E.

AU - Lau, Kenny

AU - Vasantha, Premkrishnan B.N.

AU - Sankaran, Gayatri

AU - Tan, Ron

AU - Schuster, Stephan C.

PY - 2018

Y1 - 2018

N2 - This study reveals biomass concentration and microbiome detected from settling dust samples collected from selected childcare centres (indoor and outdoor) in tropical climate with three ventilation types: centralized, split unit and natural ventilation. Dusts were collected by means of passive sampling by placing petri dishes at 2-meter height over 4 weeks. DNA was then extracted and analysed by fluorometry and metagenomic sequencing. The results indicated that there is no significant difference in DNA yield among different ventilation types and that a high variability was observed from petri dishes of the same site. The metagenomic analysis suggests that the indoor dust from the childcare centre with centralized air-con was the most different from outdoor microbiome, whereas the indoor dust from the naturally ventilated centres showed the most similarity to outdoor microbiome.

AB - This study reveals biomass concentration and microbiome detected from settling dust samples collected from selected childcare centres (indoor and outdoor) in tropical climate with three ventilation types: centralized, split unit and natural ventilation. Dusts were collected by means of passive sampling by placing petri dishes at 2-meter height over 4 weeks. DNA was then extracted and analysed by fluorometry and metagenomic sequencing. The results indicated that there is no significant difference in DNA yield among different ventilation types and that a high variability was observed from petri dishes of the same site. The metagenomic analysis suggests that the indoor dust from the childcare centre with centralized air-con was the most different from outdoor microbiome, whereas the indoor dust from the naturally ventilated centres showed the most similarity to outdoor microbiome.

KW - Air conditioning system

KW - Microbiome

KW - Passive dust collection

KW - Tropical climate bioaerosols

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M3 - Conference contribution

AN - SCOPUS:85105615800

T3 - 15th Conference of the International Society of Indoor Air Quality and Climate, INDOOR AIR 2018

BT - 15th Conference of the International Society of Indoor Air Quality and Climate, INDOOR AIR 2018

PB - International Society of Indoor Air Quality and Climate

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Y2 - 22 July 2018 through 27 July 2018

ER -

Uchida A, Luhung I, Miller D, Kolundzija S, Heinle CE, Lau K et al. The microbiome of settling dust from childcare centres in Singapore. In 15th Conference of the International Society of Indoor Air Quality and Climate, INDOOR AIR 2018. International Society of Indoor Air Quality and Climate. 2018. (15th Conference of the International Society of Indoor Air Quality and Climate, INDOOR AIR 2018).

The microbiome of settling dust from childcare centres in Singapore

Abstract

Publication series

Conference

Bibliographical note

ASJC Scopus Subject Areas

Keywords

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