The suppressor gene scl1+ of Saccharomyces cerevisiae is essential for growth

Elisabetta Baizi; Weining Chen; Etienne Capieaux; John H. McCusker; James E. Haber; André Goffeau

doi:10.1016/0378-1119(89)90113-3

The suppressor gene scl1⁺ of Saccharomyces cerevisiae is essential for growth

Elisabetta Baizi, Weining Chen, Etienne Capieaux, John H. McCusker, James E. Haber, André Goffeau^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

In Saccharomyces cerevisiae, the SCLl-1 mutation is a dominant suppressor of the cycloheximide-resistant, temperature-sensitive (ts) lethal mutation, crl3 [McCusker and Haber, Genetics 119 (1988a) 303-315]. The wild-type scl1⁺ gene was isolated by screening subclones of the 35-kb region between TRP5 and LEU1 for restoration of the ts phenotype in an SCLl-l crl3-2 strain. The sell + mRNA is about 900 nt long and encodes an open reading frame of 810 bp. The polypeptide deduced from sell + possesses a putative secretory signal peptide. The 5'-noncoding region may be under multiple controls, since it contains significant homology to the consensus sequences for the DNA-binding proteins, GCN4, GFI and, possibly, TUF. Gene disruption of sell + demonstrates that it is an essential gene.

Original language	English
Pages (from-to)	271-279
Number of pages	9
Journal	Gene
Volume	83
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(89)90113-3
Publication status	Published - Nov 30 1989
Externally published	Yes

ASJC Scopus Subject Areas

Genetics

Keywords

cycloheximide resistance
Recombinant DNA
secretory signal peptide
temperature lethality
transcription regulation

Access to Document

10.1016/0378-1119(89)90113-3

Cite this

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title = "The suppressor gene scl1+ of Saccharomyces cerevisiae is essential for growth",

abstract = "In Saccharomyces cerevisiae, the SCLl-1 mutation is a dominant suppressor of the cycloheximide-resistant, temperature-sensitive (ts) lethal mutation, crl3 [McCusker and Haber, Genetics 119 (1988a) 303-315]. The wild-type scl1+ gene was isolated by screening subclones of the 35-kb region between TRP5 and LEU1 for restoration of the ts phenotype in an SCLl-l crl3-2 strain. The sell + mRNA is about 900 nt long and encodes an open reading frame of 810 bp. The polypeptide deduced from sell + possesses a putative secretory signal peptide. The 5'-noncoding region may be under multiple controls, since it contains significant homology to the consensus sequences for the DNA-binding proteins, GCN4, GFI and, possibly, TUF. Gene disruption of sell + demonstrates that it is an essential gene.",

keywords = "cycloheximide resistance, Recombinant DNA, secretory signal peptide, temperature lethality, transcription regulation",

author = "Elisabetta Baizi and Weining Chen and Etienne Capieaux and McCusker, \{John H.\} and Haber, \{James E.\} and Andr{\'e} Goffeau",

year = "1989",

month = nov,

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doi = "10.1016/0378-1119(89)90113-3",

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T1 - The suppressor gene scl1+ of Saccharomyces cerevisiae is essential for growth

AU - Baizi, Elisabetta

AU - Chen, Weining

AU - Capieaux, Etienne

AU - McCusker, John H.

AU - Haber, James E.

AU - Goffeau, André

PY - 1989/11/30

Y1 - 1989/11/30

N2 - In Saccharomyces cerevisiae, the SCLl-1 mutation is a dominant suppressor of the cycloheximide-resistant, temperature-sensitive (ts) lethal mutation, crl3 [McCusker and Haber, Genetics 119 (1988a) 303-315]. The wild-type scl1+ gene was isolated by screening subclones of the 35-kb region between TRP5 and LEU1 for restoration of the ts phenotype in an SCLl-l crl3-2 strain. The sell + mRNA is about 900 nt long and encodes an open reading frame of 810 bp. The polypeptide deduced from sell + possesses a putative secretory signal peptide. The 5'-noncoding region may be under multiple controls, since it contains significant homology to the consensus sequences for the DNA-binding proteins, GCN4, GFI and, possibly, TUF. Gene disruption of sell + demonstrates that it is an essential gene.

AB - In Saccharomyces cerevisiae, the SCLl-1 mutation is a dominant suppressor of the cycloheximide-resistant, temperature-sensitive (ts) lethal mutation, crl3 [McCusker and Haber, Genetics 119 (1988a) 303-315]. The wild-type scl1+ gene was isolated by screening subclones of the 35-kb region between TRP5 and LEU1 for restoration of the ts phenotype in an SCLl-l crl3-2 strain. The sell + mRNA is about 900 nt long and encodes an open reading frame of 810 bp. The polypeptide deduced from sell + possesses a putative secretory signal peptide. The 5'-noncoding region may be under multiple controls, since it contains significant homology to the consensus sequences for the DNA-binding proteins, GCN4, GFI and, possibly, TUF. Gene disruption of sell + demonstrates that it is an essential gene.

KW - cycloheximide resistance

KW - Recombinant DNA

KW - secretory signal peptide

KW - temperature lethality

KW - transcription regulation

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