Three-dimensional structured illumination microscopy (3D-SIM) to dissect signaling cross-talks in motile T-cells

Seow Theng Ong; Graham D. Wright; Navin Kumar Verma

doi:10.1007/978-1-4939-9036-8_6

Three-dimensional structured illumination microscopy (3D-SIM) to dissect signaling cross-talks in motile T-cells

Seow Theng Ong^*, Graham D. Wright, Navin Kumar Verma

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Citation (Scopus)

Abstract

Visualization of signal transduction events in T-cells has always been a challenge due to their miniscule size. Recent advancement in super-resolution microscopy techniques presents many new opportunities to navigate the spatial and temporal signaling cross-talks in motile T-cells. Here, we provide technical details, optimal conditions, and critical practical considerations that need to be taken into account during cell handling, sample preparation, and image acquisition of motile T-cells for performing three-dimensional structured illumination microscopy (3D-SIM).

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	41-50
Number of pages	10
DOIs	https://doi.org/10.1007/978-1-4939-9036-8_6
Publication status	Published - 2019
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	1930
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© 2019, Springer Science+Business Media, LLC, part of Springer Nature.

ASJC Scopus Subject Areas

Molecular Biology
Genetics

Keywords

3D-SIM
Immunostaining
Super-resolution microscopy

Access to Document

10.1007/978-1-4939-9036-8_6

Cite this

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abstract = "Visualization of signal transduction events in T-cells has always been a challenge due to their miniscule size. Recent advancement in super-resolution microscopy techniques presents many new opportunities to navigate the spatial and temporal signaling cross-talks in motile T-cells. Here, we provide technical details, optimal conditions, and critical practical considerations that need to be taken into account during cell handling, sample preparation, and image acquisition of motile T-cells for performing three-dimensional structured illumination microscopy (3D-SIM).",

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year = "2019",

doi = "10.1007/978-1-4939-9036-8_6",

language = "English",

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AU - Wright, Graham D.

AU - Verma, Navin Kumar

PY - 2019

Y1 - 2019

N2 - Visualization of signal transduction events in T-cells has always been a challenge due to their miniscule size. Recent advancement in super-resolution microscopy techniques presents many new opportunities to navigate the spatial and temporal signaling cross-talks in motile T-cells. Here, we provide technical details, optimal conditions, and critical practical considerations that need to be taken into account during cell handling, sample preparation, and image acquisition of motile T-cells for performing three-dimensional structured illumination microscopy (3D-SIM).

AB - Visualization of signal transduction events in T-cells has always been a challenge due to their miniscule size. Recent advancement in super-resolution microscopy techniques presents many new opportunities to navigate the spatial and temporal signaling cross-talks in motile T-cells. Here, we provide technical details, optimal conditions, and critical practical considerations that need to be taken into account during cell handling, sample preparation, and image acquisition of motile T-cells for performing three-dimensional structured illumination microscopy (3D-SIM).

KW - 3D-SIM

KW - Immunostaining

KW - Super-resolution microscopy

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BT - Methods in Molecular Biology

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Three-dimensional structured illumination microscopy (3D-SIM) to dissect signaling cross-talks in motile T-cells

Abstract

Publication series

Bibliographical note

ASJC Scopus Subject Areas

Keywords

Access to Document

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