Towards field malaria diagnosis using surface enhanced Raman spectroscopy

Keren Chen; Aoli Xiong; Clement Yuen; Peter Preiser; Quan Liu

doi:10.1117/12.2227042

Towards field malaria diagnosis using surface enhanced Raman spectroscopy

Keren Chen, Aoli Xiong, Clement Yuen, Peter Preiser, Quan Liu

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Citation (Scopus)

Abstract

We report three strategies of surface enhanced Raman spectroscopy (SERS) for β-hematin and hemozoin detection in malaria infected human blood, which can be potentially developed for field malaria diagnosis. In the first strategy, we used silver coated magnetic nanoparticles (Fe₃O₄@Ag) in combination with an external magnetic field to enhance the Raman signal of β-hematin. Then we developed two SERS methods without the requirement of magnetic field for malaria infection diagnosis. In Method 1, silver nanoparticles were synthesized separately and then mixed with lysed blood just like in traditional SERS measurements; while in Method 2, we developed an ultrasensitive SERS method by synthesizing silver nanoparticles directly inside the parasites of Plasmodium falciparum. Method 2 can be also used to detect single parasites in the ring stage.

Original language	English
Title of host publication	Biophotonics
Subtitle of host publication	Photonic Solutions for Better Health Care V
Editors	Valery V. Tuchin, Francesco S. Pavone, Jurgen Popp, Dennis L. Matthews
Publisher	SPIE
ISBN (Electronic)	9781510601321
DOIs	https://doi.org/10.1117/12.2227042
Publication status	Published - 2016
Externally published	Yes
Event	Biophotonics: Photonic Solutions for Better Health Care V - Brussels, Belgium Duration: Apr 4 2016 → Apr 7 2016

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	9887
ISSN (Print)	0277-786X
ISSN (Electronic)	1996-756X

Conference

Conference	Biophotonics: Photonic Solutions for Better Health Care V
Country/Territory	Belgium
City	Brussels
Period	4/4/16 → 4/7/16

Bibliographical note

Publisher Copyright:
© 2016 SPIE.

ASJC Scopus Subject Areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

Keywords

Hemozoin
Malaria
Raman Spectroscopy
Silver nanoparticles
Surface enhanced Raman scattering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1117/12.2227042

Cite this

Chen, K., Xiong, A., Yuen, C., Preiser, P., & Liu, Q. (2016). Towards field malaria diagnosis using surface enhanced Raman spectroscopy. In V. V. Tuchin, F. S. Pavone, J. Popp, & D. L. Matthews (Eds.), Biophotonics: Photonic Solutions for Better Health Care V Article 98870M (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 9887). SPIE. https://doi.org/10.1117/12.2227042

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title = "Towards field malaria diagnosis using surface enhanced Raman spectroscopy",

abstract = "We report three strategies of surface enhanced Raman spectroscopy (SERS) for β-hematin and hemozoin detection in malaria infected human blood, which can be potentially developed for field malaria diagnosis. In the first strategy, we used silver coated magnetic nanoparticles (Fe3O4@Ag) in combination with an external magnetic field to enhance the Raman signal of β-hematin. Then we developed two SERS methods without the requirement of magnetic field for malaria infection diagnosis. In Method 1, silver nanoparticles were synthesized separately and then mixed with lysed blood just like in traditional SERS measurements; while in Method 2, we developed an ultrasensitive SERS method by synthesizing silver nanoparticles directly inside the parasites of Plasmodium falciparum. Method 2 can be also used to detect single parasites in the ring stage.",

keywords = "Hemozoin, Malaria, Raman Spectroscopy, Silver nanoparticles, Surface enhanced Raman scattering",

author = "Keren Chen and Aoli Xiong and Clement Yuen and Peter Preiser and Quan Liu",

note = "Publisher Copyright: {\textcopyright} 2016 SPIE.; Biophotonics: Photonic Solutions for Better Health Care V ; Conference date: 04-04-2016 Through 07-04-2016",

year = "2016",

doi = "10.1117/12.2227042",

language = "English",

series = "Proceedings of SPIE - The International Society for Optical Engineering",

publisher = "SPIE",

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}

Chen, K, Xiong, A, Yuen, C, Preiser, P & Liu, Q 2016, Towards field malaria diagnosis using surface enhanced Raman spectroscopy. in VV Tuchin, FS Pavone, J Popp & DL Matthews (eds), Biophotonics: Photonic Solutions for Better Health Care V., 98870M, Proceedings of SPIE - The International Society for Optical Engineering, vol. 9887, SPIE, Biophotonics: Photonic Solutions for Better Health Care V, Brussels, Belgium, 4/4/16. https://doi.org/10.1117/12.2227042

Towards field malaria diagnosis using surface enhanced Raman spectroscopy. / Chen, Keren; Xiong, Aoli; Yuen, Clement et al.
Biophotonics: Photonic Solutions for Better Health Care V. ed. / Valery V. Tuchin; Francesco S. Pavone; Jurgen Popp; Dennis L. Matthews. SPIE, 2016. 98870M (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 9887).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Towards field malaria diagnosis using surface enhanced Raman spectroscopy

AU - Chen, Keren

AU - Xiong, Aoli

AU - Yuen, Clement

AU - Preiser, Peter

AU - Liu, Quan

PY - 2016

Y1 - 2016

N2 - We report three strategies of surface enhanced Raman spectroscopy (SERS) for β-hematin and hemozoin detection in malaria infected human blood, which can be potentially developed for field malaria diagnosis. In the first strategy, we used silver coated magnetic nanoparticles (Fe3O4@Ag) in combination with an external magnetic field to enhance the Raman signal of β-hematin. Then we developed two SERS methods without the requirement of magnetic field for malaria infection diagnosis. In Method 1, silver nanoparticles were synthesized separately and then mixed with lysed blood just like in traditional SERS measurements; while in Method 2, we developed an ultrasensitive SERS method by synthesizing silver nanoparticles directly inside the parasites of Plasmodium falciparum. Method 2 can be also used to detect single parasites in the ring stage.

AB - We report three strategies of surface enhanced Raman spectroscopy (SERS) for β-hematin and hemozoin detection in malaria infected human blood, which can be potentially developed for field malaria diagnosis. In the first strategy, we used silver coated magnetic nanoparticles (Fe3O4@Ag) in combination with an external magnetic field to enhance the Raman signal of β-hematin. Then we developed two SERS methods without the requirement of magnetic field for malaria infection diagnosis. In Method 1, silver nanoparticles were synthesized separately and then mixed with lysed blood just like in traditional SERS measurements; while in Method 2, we developed an ultrasensitive SERS method by synthesizing silver nanoparticles directly inside the parasites of Plasmodium falciparum. Method 2 can be also used to detect single parasites in the ring stage.

KW - Hemozoin

KW - Malaria

KW - Raman Spectroscopy

KW - Silver nanoparticles

KW - Surface enhanced Raman scattering

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A2 - Pavone, Francesco S.

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ER -

Towards field malaria diagnosis using surface enhanced Raman spectroscopy

Abstract

Publication series

Conference

Bibliographical note

ASJC Scopus Subject Areas

Keywords

UN SDGs

Access to Document

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