TY - JOUR
T1 - Two-component regulatory system involved in transcriptional control of heavy-metal homoeostasis in Alcaligenes eutrophus
AU - Van Der Lelie, Daniel
AU - Schwuchow, Tanja
AU - Schwidetzky, Uta
AU - Wuertz, Stefan
AU - Baeyens, Wilfried
AU - Mergeay, Max
AU - Nies, Dietrich H.
PY - 1997
Y1 - 1997
N2 - The czc determinant, which mediates resistance to Co2+, Zn2+ and Cd2+ in Alcaligenes eutrophus CH34 by cation efflux, is regulated by a two component regulatory system composed of the sensor histidine kinase CzcS and the response activator CzcR (in addition to other components previously described). Regulatory genes are arranged in an upstream regulatory region (DRR) and a downstream regulatory region (DRR). Transcription of czcCBA and of the DRR was regulated by heavy-metal cations. DNA sequencing of the region downstream of czcD revealed the presence of the czcR and czcS genes which together with czcD form the DRR. Regulation of the DRR was studied with a czcD::lacZ translational fusion and a czcS::lux transcriptional fusion. Expression of both genes is also regulated by heavy metals. The genes of the DRR yielded three mRNAs of approx. 1200, 500 and 200 nucleotides, respectively. The genes czcCBA for the cation/proton antiporter CzcCBA were transcribed by one operon as a transcript of 6200 nucleotides.
AB - The czc determinant, which mediates resistance to Co2+, Zn2+ and Cd2+ in Alcaligenes eutrophus CH34 by cation efflux, is regulated by a two component regulatory system composed of the sensor histidine kinase CzcS and the response activator CzcR (in addition to other components previously described). Regulatory genes are arranged in an upstream regulatory region (DRR) and a downstream regulatory region (DRR). Transcription of czcCBA and of the DRR was regulated by heavy-metal cations. DNA sequencing of the region downstream of czcD revealed the presence of the czcR and czcS genes which together with czcD form the DRR. Regulation of the DRR was studied with a czcD::lacZ translational fusion and a czcS::lux transcriptional fusion. Expression of both genes is also regulated by heavy metals. The genes of the DRR yielded three mRNAs of approx. 1200, 500 and 200 nucleotides, respectively. The genes czcCBA for the cation/proton antiporter CzcCBA were transcribed by one operon as a transcript of 6200 nucleotides.
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U2 - 10.1046/j.1365-2958.1997.d01-1866.x
DO - 10.1046/j.1365-2958.1997.d01-1866.x
M3 - Article
C2 - 9044283
AN - SCOPUS:0031035708
SN - 0950-382X
VL - 23
SP - 493
EP - 503
JO - Molecular Microbiology
JF - Molecular Microbiology
IS - 3
ER -