Whole-genome shotgun sequencing of mitochondria from ancient hair shafts

M. Thomas P. Gilbert; Lynn P. Tomsho; Snjezana Rendulic; Michael Packard; Daniela I. Drautz; Andrei Sher; Alexei Tikhonov; Love Dalén; Tatyana Kuznetsova; Pavel Kosintsev; Paula F. Campos; Thomas Higham; Matthew J. Collins; Andrew S. Wilson; Fyodor Shidlovskiy; Bernard Buigues; Per G.P. Ericson; Mietje Germonpré; Anders Götherström; Paola Iacumin; Vladimir Nikolaev; Malgosia Nowak-Kemp; Eske Willerslev; James R. Knight; Gerard P. Irzyk; Clotilde S. Perbost; Karin M. Fredrikson; Timothy T. Harkins; Sharon Sheridan; Webb Miller; Stephan C. Schuster

doi:10.1126/science.1146971

Whole-genome shotgun sequencing of mitochondria from ancient hair shafts

M. Thomas P. Gilbert, Lynn P. Tomsho, Snjezana Rendulic, Michael Packard, Daniela I. Drautz, Andrei Sher, Alexei Tikhonov, Love Dalén, Tatyana Kuznetsova, Pavel Kosintsev, Paula F. Campos, Thomas Higham, Matthew J. Collins, Andrew S. Wilson, Fyodor Shidlovskiy, Bernard Buigues, Per G.P. Ericson, Mietje Germonpré, Anders Götherström, Paola IacuminVladimir Nikolaev, Malgosia Nowak-Kemp, Eske Willerslev, James R. Knight, Gerard P. Irzyk, Clotilde S. Perbost, Karin M. Fredrikson, Timothy T. Harkins, Sharon Sheridan, Webb Miller^*, Stephan C. Schuster

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

209 Citations (Scopus)

Abstract

Although the application of sequencing-by-synthesis techniques to DNA extracted from bones has revolutionized the study of ancient DNA, it has been plagued by large fractions of contaminating environmental DNA. The genetic analyses of hair shafts could be a solution: We present 10 previously unexamined Siberian mammoth (Mammuthus primigenius) mitochondrial genomes, sequenced with up to 48-fold coverage. The observed levels of damage-derived sequencing errors were lower than those observed in previously published frozen bone samples, even though one of the specimens was >50,000 ¹⁴C years old and another had been stored for 200 years at room temperature. The method therefore sets the stage for molecular-genetic analysis of museum collections.

Original language	English
Pages (from-to)	1927-1930
Number of pages	4
Journal	Science
Volume	317
Issue number	5846
DOIs	https://doi.org/10.1126/science.1146971
Publication status	Published - Sept 28 2007
Externally published	Yes

ASJC Scopus Subject Areas

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Gilbert, M. T. P., Tomsho, L. P., Rendulic, S., Packard, M., Drautz, D. I., Sher, A., Tikhonov, A., Dalén, L., Kuznetsova, T., Kosintsev, P., Campos, P. F., Higham, T., Collins, M. J., Wilson, A. S., Shidlovskiy, F., Buigues, B., Ericson, P. G. P., Germonpré, M., Götherström, A., ... Schuster, S. C. (2007). Whole-genome shotgun sequencing of mitochondria from ancient hair shafts. Science, 317(5846), 1927-1930. https://doi.org/10.1126/science.1146971

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title = "Whole-genome shotgun sequencing of mitochondria from ancient hair shafts",

abstract = "Although the application of sequencing-by-synthesis techniques to DNA extracted from bones has revolutionized the study of ancient DNA, it has been plagued by large fractions of contaminating environmental DNA. The genetic analyses of hair shafts could be a solution: We present 10 previously unexamined Siberian mammoth (Mammuthus primigenius) mitochondrial genomes, sequenced with up to 48-fold coverage. The observed levels of damage-derived sequencing errors were lower than those observed in previously published frozen bone samples, even though one of the specimens was >50,000 14C years old and another had been stored for 200 years at room temperature. The method therefore sets the stage for molecular-genetic analysis of museum collections.",

author = "Gilbert, {M. Thomas P.} and Tomsho, {Lynn P.} and Snjezana Rendulic and Michael Packard and Drautz, {Daniela I.} and Andrei Sher and Alexei Tikhonov and Love Dal{\'e}n and Tatyana Kuznetsova and Pavel Kosintsev and Campos, {Paula F.} and Thomas Higham and Collins, {Matthew J.} and Wilson, {Andrew S.} and Fyodor Shidlovskiy and Bernard Buigues and Ericson, {Per G.P.} and Mietje Germonpr{\'e} and Anders G{\"o}therstr{\"o}m and Paola Iacumin and Vladimir Nikolaev and Malgosia Nowak-Kemp and Eske Willerslev and Knight, {James R.} and Irzyk, {Gerard P.} and Perbost, {Clotilde S.} and Fredrikson, {Karin M.} and Harkins, {Timothy T.} and Sharon Sheridan and Webb Miller and Schuster, {Stephan C.}",

year = "2007",

month = sep,

day = "28",

doi = "10.1126/science.1146971",

language = "English",

volume = "317",

pages = "1927--1930",

journal = "Science",

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Gilbert, MTP, Tomsho, LP, Rendulic, S, Packard, M, Drautz, DI, Sher, A, Tikhonov, A, Dalén, L, Kuznetsova, T, Kosintsev, P, Campos, PF, Higham, T, Collins, MJ, Wilson, AS, Shidlovskiy, F, Buigues, B, Ericson, PGP, Germonpré, M, Götherström, A, Iacumin, P, Nikolaev, V, Nowak-Kemp, M, Willerslev, E, Knight, JR, Irzyk, GP, Perbost, CS, Fredrikson, KM, Harkins, TT, Sheridan, S, Miller, W & Schuster, SC 2007, 'Whole-genome shotgun sequencing of mitochondria from ancient hair shafts', Science, vol. 317, no. 5846, pp. 1927-1930. https://doi.org/10.1126/science.1146971

TY - JOUR

T1 - Whole-genome shotgun sequencing of mitochondria from ancient hair shafts

AU - Gilbert, M. Thomas P.

AU - Tomsho, Lynn P.

AU - Rendulic, Snjezana

AU - Packard, Michael

AU - Drautz, Daniela I.

AU - Sher, Andrei

AU - Tikhonov, Alexei

AU - Dalén, Love

AU - Kuznetsova, Tatyana

AU - Kosintsev, Pavel

AU - Campos, Paula F.

AU - Higham, Thomas

AU - Collins, Matthew J.

AU - Wilson, Andrew S.

AU - Shidlovskiy, Fyodor

AU - Buigues, Bernard

AU - Ericson, Per G.P.

AU - Germonpré, Mietje

AU - Götherström, Anders

AU - Iacumin, Paola

AU - Nikolaev, Vladimir

AU - Nowak-Kemp, Malgosia

AU - Willerslev, Eske

AU - Knight, James R.

AU - Irzyk, Gerard P.

AU - Perbost, Clotilde S.

AU - Fredrikson, Karin M.

AU - Harkins, Timothy T.

AU - Sheridan, Sharon

AU - Miller, Webb

AU - Schuster, Stephan C.

PY - 2007/9/28

Y1 - 2007/9/28

N2 - Although the application of sequencing-by-synthesis techniques to DNA extracted from bones has revolutionized the study of ancient DNA, it has been plagued by large fractions of contaminating environmental DNA. The genetic analyses of hair shafts could be a solution: We present 10 previously unexamined Siberian mammoth (Mammuthus primigenius) mitochondrial genomes, sequenced with up to 48-fold coverage. The observed levels of damage-derived sequencing errors were lower than those observed in previously published frozen bone samples, even though one of the specimens was >50,000 14C years old and another had been stored for 200 years at room temperature. The method therefore sets the stage for molecular-genetic analysis of museum collections.

AB - Although the application of sequencing-by-synthesis techniques to DNA extracted from bones has revolutionized the study of ancient DNA, it has been plagued by large fractions of contaminating environmental DNA. The genetic analyses of hair shafts could be a solution: We present 10 previously unexamined Siberian mammoth (Mammuthus primigenius) mitochondrial genomes, sequenced with up to 48-fold coverage. The observed levels of damage-derived sequencing errors were lower than those observed in previously published frozen bone samples, even though one of the specimens was >50,000 14C years old and another had been stored for 200 years at room temperature. The method therefore sets the stage for molecular-genetic analysis of museum collections.

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SN - 0036-8075

VL - 317

SP - 1927

EP - 1930

JO - Science

JF - Science

IS - 5846

ER -

Whole-genome shotgun sequencing of mitochondria from ancient hair shafts

Abstract

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