TY - JOUR
T1 - A Three-Photon Active Organic Fluorophore for Deep Tissue Ratiometric Imaging of Intracellular Divalent Zinc
AU - Philips, Divya Susan
AU - Sreejith, Sivaramapanicker
AU - He, Tingchao
AU - Menon, Nishanth Venugopal
AU - Anees, Palapuravan
AU - Mathew, Jomon
AU - Sajikumar, Sreedharan
AU - Kang, Yuejun
AU - Stuparu, Mihaiela Corina
AU - Sun, Handong
AU - Zhao, Yanli
AU - Ajayaghosh, Ayyappanpillai
N1 - Publisher Copyright:
© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
PY - 2016/5/20
Y1 - 2016/5/20
N2 - Deep tissue bioimaging with three-photon (3P) excitation using near-infrared (NIR) light in the second IR window (1.0-1.4 μm) could provide high resolution images with an improved signal-to-noise ratio. Herein, we report a photostable and nontoxic 3P excitable donor-π-acceptor system (GMP) having 3P cross-section (σ3) of 1.78×10-80 cm6 s2 photon-2 and action cross-section (σ3η3) of 2.31×10-81 cm6 s2 photon-2, which provides ratiometric fluorescence response with divalent zinc ions in aqueous conditions. The probe signals the Zn2+ binding at 530 and 600 nm, respectively, upon 1150 nm excitation with enhanced σ3 of 1.85×10-80 cm6 s2 photon-2 and σ3η3 of 3.33×10-81 cm6 s2 photon-2. The application of this probe is demonstrated for ratiometric 3P imaging of Zn2+ in vitro using HuH-7 cell lines. Furthermore, the Zn2+ concentration in rat hippocampal slices was imaged at 1150 nm excitation after incubation with GMP, illustrating its potential as a 3P ratiometric probe for deep tissue Zn2+ ion imaging. Shining bright: A new three-photon (3P) active organic fluorophore with a high 3P absorption cross section close to that of inorganic-semiconductor-based systems is reported, which allows deep tissue ratiometric imaging of Zn2+ in hippocampal slices.
AB - Deep tissue bioimaging with three-photon (3P) excitation using near-infrared (NIR) light in the second IR window (1.0-1.4 μm) could provide high resolution images with an improved signal-to-noise ratio. Herein, we report a photostable and nontoxic 3P excitable donor-π-acceptor system (GMP) having 3P cross-section (σ3) of 1.78×10-80 cm6 s2 photon-2 and action cross-section (σ3η3) of 2.31×10-81 cm6 s2 photon-2, which provides ratiometric fluorescence response with divalent zinc ions in aqueous conditions. The probe signals the Zn2+ binding at 530 and 600 nm, respectively, upon 1150 nm excitation with enhanced σ3 of 1.85×10-80 cm6 s2 photon-2 and σ3η3 of 3.33×10-81 cm6 s2 photon-2. The application of this probe is demonstrated for ratiometric 3P imaging of Zn2+ in vitro using HuH-7 cell lines. Furthermore, the Zn2+ concentration in rat hippocampal slices was imaged at 1150 nm excitation after incubation with GMP, illustrating its potential as a 3P ratiometric probe for deep tissue Zn2+ ion imaging. Shining bright: A new three-photon (3P) active organic fluorophore with a high 3P absorption cross section close to that of inorganic-semiconductor-based systems is reported, which allows deep tissue ratiometric imaging of Zn2+ in hippocampal slices.
KW - fluorescent probes
KW - hippocampus
KW - imaging agents
KW - three-photon probes
KW - zinc
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U2 - 10.1002/asia.201600170
DO - 10.1002/asia.201600170
M3 - Article
C2 - 26991763
AN - SCOPUS:84979469466
SN - 1861-4728
VL - 11
SP - 1523
EP - 1527
JO - Chemistry - An Asian Journal
JF - Chemistry - An Asian Journal
IS - 10
ER -