Generation of four H1 hESC sublines carrying a hemizygous knock-out/mutant MECP2

Ruizhu Zeng; Harwin Sidik; Kim S. Robinson; Franklin L. Zhong; Bruno Reversade; Mahmoud A. Pouladi

doi:10.1016/j.scr.2019.101533

Generation of four H1 hESC sublines carrying a hemizygous knock-out/mutant MECP2

Ruizhu Zeng, Harwin Sidik, Kim S. Robinson, Franklin L. Zhong, Bruno Reversade, Mahmoud A. Pouladi^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Rett syndrome (RTT) is a childhood neurodevelopmental disorder caused by mutations in MECP2. To study the molecular mechanisms underlying RTT, four sublines of H1 hESCs were generated, carrying a hemizygous knockout or mutant allele of MECP2. Exons 3 and 4 of MECP2 were targeted using the CRISPR/Cas9 nuclease system.

Original language	English
Article number	101533
Journal	Stem Cell Research
Volume	40
DOIs	https://doi.org/10.1016/j.scr.2019.101533
Publication status	Published - Oct 2019
Externally published	Yes

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Publisher Copyright:
© 2019 The Authors

ASJC Scopus Subject Areas

Developmental Biology
Cell Biology

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title = "Generation of four H1 hESC sublines carrying a hemizygous knock-out/mutant MECP2",

abstract = "Rett syndrome (RTT) is a childhood neurodevelopmental disorder caused by mutations in MECP2. To study the molecular mechanisms underlying RTT, four sublines of H1 hESCs were generated, carrying a hemizygous knockout or mutant allele of MECP2. Exons 3 and 4 of MECP2 were targeted using the CRISPR/Cas9 nuclease system.",

author = "Ruizhu Zeng and Harwin Sidik and Robinson, {Kim S.} and Zhong, {Franklin L.} and Bruno Reversade and Pouladi, {Mahmoud A.}",

note = "Publisher Copyright: {\textcopyright} 2019 The Authors",

year = "2019",

month = oct,

doi = "10.1016/j.scr.2019.101533",

language = "English",

volume = "40",

journal = "Stem Cell Research",

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T1 - Generation of four H1 hESC sublines carrying a hemizygous knock-out/mutant MECP2

AU - Zeng, Ruizhu

AU - Sidik, Harwin

AU - Robinson, Kim S.

AU - Zhong, Franklin L.

AU - Reversade, Bruno

AU - Pouladi, Mahmoud A.

PY - 2019/10

Y1 - 2019/10

N2 - Rett syndrome (RTT) is a childhood neurodevelopmental disorder caused by mutations in MECP2. To study the molecular mechanisms underlying RTT, four sublines of H1 hESCs were generated, carrying a hemizygous knockout or mutant allele of MECP2. Exons 3 and 4 of MECP2 were targeted using the CRISPR/Cas9 nuclease system.

AB - Rett syndrome (RTT) is a childhood neurodevelopmental disorder caused by mutations in MECP2. To study the molecular mechanisms underlying RTT, four sublines of H1 hESCs were generated, carrying a hemizygous knockout or mutant allele of MECP2. Exons 3 and 4 of MECP2 were targeted using the CRISPR/Cas9 nuclease system.

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VL - 40

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JF - Stem Cell Research

M1 - 101533

ER -

Generation of four H1 hESC sublines carrying a hemizygous knock-out/mutant MECP2

Abstract

Bibliographical note

ASJC Scopus Subject Areas

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